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Monoclonal antibodies for the detection and quantitation of the endogenous plant growth regulator, abscisic acid
Monoclonal antibodies (mAB) have been produced which recognize the physiologically active 2‐cis‐(S‐form of the endogenous plant growth regulator, abscisic acid (ABA). Cross‐reaction with theExpand
Hyperaccumulation, complexation and distribution of nickel in Sebertia acuminata.
A repellent effect of the plant sap was observed on the fruit fly Drosophila melanogaster indicating that in hyperaccumulating plants nickel functions as an agent to prevent predation. Expand
Instability of Indole Alkaloid Production in Catharanthus roseus Cell Suspension Cultures.
Using serpentine fluorescence as an indicator of alkaloid production in cultured CATHARANTHUS ROSEUS cells, 6 cell lines producing alkaloid in excess of 300 mg/l were selected from more than 2 x 10Expand
Benzylisoquinoline Biosynthesis by Cultivated Plant Cells and Isolated Enzymes
It could be demonstrated that the transport of these compounds into vacuoles of Fumaria capreolata does not proceed by an ion-trap mechanism, but rather involves an active ATP-requiring, stereospecific carrier system acting only on the (S)and not on the @?)-enantiomer. Expand
A highly selective alkaloid uptake system in vacuoles of higher plants
Transport of alkaloids across the tonoplast into the vacuolar space has been characterized as an active, engergy-requiring mechanism, which is sensitive to the temperature and pH of the surrounding medium and inhibited by N,N′-dicyclohexylcarbodiimid and Cu2+. Expand
S-adenosyl-l-methionine:(S)-coclaurine-N-methyltransferase from Tinospora cordifolia
Abstract A survey of eight plant cell cultures belonging to four isoquinoline alkaloid producing plant families revealed that norcoclaurine is, in the presence of S- adenosyl- l -methionine (SAM),Expand
Biosynthesis of cyclic bis(bibenzyls) in Marchantia polymorpha
Abstract Using sterile thallus tissue of Marchantia polymorpha , the biosynthesis of the cyclic bis(bibenzyl) marchantin A was investigated. The synthesis of the radioactively and 13 C-labelledExpand
Biosynthesis of Erythrina alkaloids in Erythrina crista-galli.
Feeding of [1-13C]-labelled (S)-norreticuline with subsequent NMR spectroscopy demonstrated that the resulting erythraline was exclusively labelled at position C-10, suggesting that a coclaurine-norretsinomenine pathway is operative in Erythrina alkaloid formation. Expand
Enzyme immunoassay for the quantitative determination of galanthamine.
The preliminary results demonstrate that the galanthamine-specific enzyme immunoassay can be a useful tool in medicinal plant breeding, for screening programs, as well as for taxonomic and pharmacokinetic studies. Expand
The biosynthesis of lunarine in seeds of Lunaria annua
Using an optimized precursor feeding system, the biosynthesis of the alkaloid lunarine in Lunaria annua seeds was investigated and a cytochrome P-450 enzyme might be responsible for the phenol-oxidative coupling of N 1 , N 10 -bis yielding the hexahydrodibenzofuran ring of lunarine. Expand