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Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposons.
TLDR
Small bacteriophage Mu transposable elements containing the lac operon structural genes were constructed to facilitate the isolation and use of Mu insertions and lac gene fusions and were found to be distributed around the plasmid, but insertions in certain regions occurred more frequently than in others. Expand
Keeping the eIF2 alpha kinase Gcn2 in check.
TLDR
This review provides a critical evaluation of the current knowledge on mechanisms controlling Gcn2 activation or activity and suggests that the current level of knowledge on these mechanisms is inadequate. Expand
Novel membrane-bound eIF2alpha kinase in the flagellar pocket of Trypanosoma brucei.
TLDR
It is shown that TbeIF2K2 is a transmembrane glycoprotein expressed both in procyclic and in bloodstream forms, and its location suggests a relevance in sensing protein or nutrient transport in T. brucei, an organism that relies heavily on posttranscriptional regulatory mechanisms to control gene expression in different environmental conditions. Expand
Prion protein interaction with stress-inducible protein 1 enhances neuronal protein synthesis via mTOR
TLDR
It is demonstrated that protein synthesis in neurons is enhanced via PrPC interaction with stress-inducible protein 1 (STI1) and that neuroprotection and neuritogenesis mediated by PrPC–STI 1 engagement are dependent upon the increased protein synthesis mediated by PI3K-mTOR signaling. Expand
IMPACT, a Protein Preferentially Expressed in the Mouse Brain, Binds GCN1 and Inhibits GCN2 Activation*
TLDR
Evidence is presented that IMPACT is the functional counterpart of YIH1, an inhibitor of GCN2 that ensures constant high levels of translation and low levels of ATF4 and CHOP in specific neuronal cells under amino acid starvation conditions. Expand
Phosphorylation of translation initiation factor eIF2α in the brain during pilocarpine-induced status epilepticus in mice
TLDR
The results indicate that inhibition of protein synthesis in experimental models of epilepsy might be due to low levels of eIF2-GTP caused by the phosphorylated subunit of translation initiation factor 2 (eIF2alpha), and suggest that translational control may contribute to cell fate in the affected areas. Expand
Biophysical characterization of Gir2, a highly acidic protein of Saccharomyces cerevisiae with anomalous electrophoretic behavior.
TLDR
It is suggested that Gir2 is a representative of the growing group of "natively unfolded" proteins, as determined by CD analysis, which is thermostable and lacks extensive structure. Expand
Novel Membrane-Bound eIF2α Kinase in the Flagellar Pocket of Trypanosoma brucei
TLDR
It is shown here that TbeIF2K2 is a transmembrane glycoprotein expressed both in procyclic and in bloodstream forms, and this is the first membrane-associated eIF2α kinase described in unicellular eukaryotes. Expand
Evidence That Eukaryotic Translation Elongation Factor 1A (eEF1A) Binds the Gcn2 Protein C Terminus and Inhibits Gcn2 Activity*♦
TLDR
This work has proposed a model for sensing starvation, in which Gcn1 and Gcn2 are tethered to the ribosome, and EEF1A is directly involved in delivering uncharged tRNAs from the A-site to GCN2 for its subsequent activation. Expand
The β Subunit of Eukaryotic Translation Initiation Factor 2 Binds mRNA through the Lysine Repeats and a Region Comprising the C2-C2 Motif
TLDR
It is shown that only one run of lysine residues is sufficient for the in vivo function of eIF2β, probably through charge interaction, since its replacement by arginines did not impair cell viability, whereas substitution for alanines resulted in inviable cells. Expand
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