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Efficient octopine Ti plasmid-derived vectors for Agrobacterium- mediated gene transfer to plants
TLDR
Strains carrying the resulting cointegrated plasmids transfer and integrate T-DNA into the genome of tobacco protoplasts, and transformed tobacco calli are readily selected as resistant to kanamycin.
The Cape element in the Afrotemperate flora: from Cape to Cairo?
TLDR
This work reconstructs the historical biogeography of the four lineages of Disa, Irideae p.p., the Pentaschistis clade and Restionaceae, and finds that tropical taxa are nested within a predominantly Cape clade.
Extinction Risk and Diversification Are Linked in a Plant Biodiversity Hotspot
Plant extinction risks in the Cape, South Africa differ from those for vertebrates worldwide, with young and fast-evolving plant lineages marching towards extinction at the fastest rate, but
Molecular phylogenetics of Vandeae (Orchidaceae) and the evolution of leaflessness.
TLDR
A change in leaf morphology, monopodial growth habit, and aeration complexes for gas exchange in photosynthetic roots seem to be the most important characters in making the evolutionary transition to leaflessness.
Estimating the age of fire in the Cape flora of South Africa from an orchid phylogeny
TLDR
The presence of two obligate post-fire flowering clades in the orchid genus Disa is exploited, in conjunction with a robust, well-sampled and dated molecular phylogeny, to estimate the age by which fire must have been present, indicating that summer drought, the fire regime and the fynbos vegetation are several million years older than currently suggested.
Woody plant communities of isolated Afromontane cloud forests in Taita Hills, Kenya
In the Taita Hills in southern Kenya, remnants of the original Afromontane forest vegetation are restricted to isolated mountain peaks. To assess the level of degradation and the need for forest
Comparative vegetative anatomy and systematics of the angraecoids (Vandeae, Orchidaceae) with an emphasis on the leafless habit
The vegetative anatomy and morphology of 142 species of the angraecoid orchids (Angraecinae + Aerangidinae) and 18 species of Aeridinae were examined using light and scanning electron microscopy.
A molecular phylogeny reveals paraphyly of the large genus Eulophia (Orchidaceae): A case for the reinstatement of Orthochilus
TLDR
This work argues for retaining Acrolophia and Oeceoclades, and splitting Eulophia into two genera on the basis of evidence that each of these four clades is genetically and morphologically distinct.
The conservation status of Saintpaulia
introduction This paper is a preliminary assessment of the conservation status of Saintpaulia H. Wendl. in both the wild and in cultivation. The genus Saintpaulia has been promoted as a botanical
T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis.
TLDR
It is confirmed by DNA hybridization that tumor lines obtained after infection of this plant by Agrobacterium strains harboring wild-type nopaline and octopine tumor-inducing plasmids are stably transformed and contain transferred DNA (T-DNA) segments identical to the T-DNA found in dicotyledonous plants.
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