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Red- and green-emitting firefly luciferase mutants for bioluminescent reporter applications.
Light emission from the North American firefly Photinus pyralis, which emits yellow-green (557-nm) light, is widely believed to be the most efficient bioluminescence system known, making thisExpand
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Red-emitting luciferases for bioluminescence reporter and imaging applications.
North American firefly Photinus pyralis luciferase, which emits yellow-green light (557nm), has been adapted for a variety of applications, including gene reporter assays, whole-cell biosensorExpand
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The role of lysine 529, a conserved residue of the acyl-adenylate-forming enzyme superfamily, in firefly luciferase.
Firefly luciferase catalyzes the highly efficient emission of yellow-green light from the substrates luciferin, Mg-ATP, and oxygen in a two-step process. The enzyme first catalyzes the adenylation ofExpand
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Site-directed mutagenesis of histidine 245 in firefly luciferase: a proposed model of the active site.
Firefly luciferase catalyzes the highly efficient emission of yellow-green light from substrate luciferin by a sequence of reactions that require Mg-ATP and molecular oxygen. We previously reportedExpand
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Mutagenesis evidence that the partial reactions of firefly bioluminescence are catalyzed by different conformations of the luciferase C-terminal domain.
Firefly luciferase catalyzes two sequential partial reactions resulting in the emission of light. The enzyme first catalyzes the adenylation of substrate luciferin with Mg-ATP followed by theExpand
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Crystal structure of firefly luciferase in a second catalytic conformation supports a domain alternation mechanism.
Beetle luciferases catalyze a two-step reaction that includes the initial adenylation of the luciferin substrate, followed by an oxidative decarboxylation that ultimately produces light. Evidence forExpand
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A mutagenesis study of the putative luciferin binding site residues of firefly luciferase.
Firefly luciferase catalyzes the highly efficient emission of yellow-green light from substrate firefly luciferin by a sequence of reactions that require Mg-ATP and molecular oxygen. We hadExpand
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An alternative mechanism of bioluminescence color determination in firefly luciferase.
Beetle luciferases (including those of the firefly) use the same luciferin substrate to naturally display light ranging in color from green (lambda(max) approximately 530 nm) to red (lambda(max)Expand
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Mutation of a Protease-sensitive Region in Firefly Luciferase Alters Light Emission Properties*
Luciferase (EC 1.13.12.7) from the North American firefly, Photinus pyralis, is widely used as a reporter enzyme in cell biology. One of its distinctive properties is a pronounced susceptibility toExpand
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Thermostable red and green light-producing firefly luciferase mutants for bioluminescent reporter applications.
Light emission from the North American firefly Photinus pyralis, which emits yellow-green (557-nm) light, is widely believed to be the most efficient bioluminescence system known, making thisExpand
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