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The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle
Genetic evidence indicated that a mutant lacking all three chitin synthases was inviable; this was confirmed by constructing a triple mutant rescued by a plasmid carrying a CHS2 gene under control of a GAL1 promoter.
Visualization of Melanosome Dynamics within Wild-Type and Dilute Melanocytes Suggests a Paradigm for Myosin V Function In Vivo
It is concluded that long-range, bidirectional, microtubule-dependent melanosome movements, coupled with actomyosin Va–dependent capture of melanosomes in the periphery, is the predominant mechanism responsible for the centrifugal transport and peripheral accumulation of melanOSomes in mouse melanocytes.
In budding yeast, contraction of the actomyosin ring and formation of the primary septum at cytokinesis depend on each other.
- Martin Schmidt, B. Bowers, Archana Varma, D. Roh, E. Cabib
- BiologyJournal of cell science
- 15 January 2002
Both chs2 and myo1 mutants show abnormalities in budding pattern and a decrease in the level of certain proteins associated with budding, such as Bud3p, Bud4p and Spa2p, and for the interdependence between actomyosin ring contraction and primary septum formation are discussed.
Direct DNA binding by Brca1
- T. Paull, D. Cortez, B. Bowers, S. Elledge, M. Gellert
- BiologyProceedings of the National Academy of Sciences…
- 15 May 2001
It is demonstrated that recombinant human Brca1 protein binds strongly to DNA, an activity conferred by a domain in the center of the BrCA1 polypeptide that inhibits the nucleolytic activities of the Mre11/Rad50/Nbs1 complex.
THE FINE STRUCTURE OF ACANTHAMOEBA CASTELLANII (NEFF STRAIN)
The volume fractions of lipid droplets, Golgi complex, mitochondria, digestive vacuoles, and autolysosomes have been determined at different stages of encystment by stereological analysis of electron micrographs.
Sequence and structure of Clp P, the proteolytic component of the ATP-dependent Clp protease of Escherichia coli.
Developmentally regulated expression of a Giardia lamblia cyst wall protein gene
The protozoan Giardia lamblia is an obligate parasite of the mammalian small intestine. We studied the expression of a gene that encodes a protein component of the cyst wall, a complex structure…
Synthesis of the yeast cell wall and its regulation.
This paper presents a meta-analysis of cell wall synthesis after Cytokinesis and discusses the role of cdc genes, chitin, and other factors related to cell cycle events.
Identification of a Novel Giardia lamblia Cyst Wall Protein with Leucine-rich Repeats
- H. Luján, M. Mowatt, J. Conrad, B. Bowers, T. Nash
- BiologyThe Journal of Biological Chemistry
- 8 December 1995
This work cloned the gene that encodes a novel 39-kDa cyst wall protein, CWP2, which has an extremely basic 121-residue COOH-terminal extension that might be involved in the sorting of these proteins to the secretory granules.
Effect of ADP and Ionic Strength on the Kinetic and Motile Properties of Recombinant Mouse Myosin V*
- Fei Wang, Lin X. Chen, J. Sellers
- Biology, ChemistryThe Journal of Biological Chemistry
- 11 February 2000
The high affinity for actin and the slow rate of ADP release helps the myosin head to remain attached to actin for a large fraction of each ATPase cycle and allows actin filaments to be moved by only a few myosIn V molecules in vitro.