The genome of Chenopodium quinoa
The assembly of a high-quality, chromosome-scale reference genome sequence for quinoa was produced using single-molecule real-time sequencing in combination with optical, chromosomes-contact and genetic maps, which facilitated the identification of the transcription factor likely to control the production of anti-nutritional triterpenoid saponins found in quinoa seeds.
The response of the maize nitrate transport system to nitrogen demand and supply across the lifecycle.
New insight is offered into the control of NO3- uptake by both plant developmental processes and N availability, and key control points that may be targeted by future plant improvement programmes to enhance N uptake relative to availability and/or demand are identified.
Mass spectrometry imaging for plant biology: a review
- B. Boughton, Dinaiz Thinagaran, Daniel Sarabia, A. Bacic, U. Roessner
- Biology, Environmental SciencePhytochemistry Reviews
- 13 October 2015
Recent advances in plant-based mass spectrometry imaging, general aspects of instrumentation, analytical approaches, sample preparation and the current trends in respective plant research are covered.
Comprehensive profiling and quantitation of amine group containing metabolites.
The stability of derivatized amines was found to be variable with oxidatively labile derivatives rapidly degrading, and with the inclusion of antioxidant and reducing agents, tris(2-carboxyethyl)-phosphine and ascorbic acid, degradation was significantly decreased, allowing reproducible identification and quantification of amine compounds in large sample sets.
Postnatal stability, tissue, and time specific effects of AHRR methylation change in response to maternal smoking in pregnancy
- B. Novaković, J. Ryan, N. Pereira, B. Boughton, J. Craig, R. Saffery
- Medicine, BiologyEpigenetics
- 1 March 2014
The evidence for postnatal stability of smoking-induced epigenetic change supports a role for epigenetics as a mediator of long-term effects of specific in utero exposures in humans.
Characterization and Identification of Dityrosine Cross-Linked Peptides Using Tandem Mass Spectrometry.
The dityrosine cross-linked residues in human hemoglobin and α-synuclein under oxidative conditions are reported, for the first time, to open up the potential for automated analysis of this naturally occurring post-translation modification in neurodegenerative diseases as well as other pathological conditions.
Conformationally constrained diketopimelic acid analogues as inhibitors of dihydrodipicolinate synthase.
Review of recent developments in GC–MS approaches to metabolomics-based research
Specific emphasis is given to the key steps within the GC–MS workflow that those new to this field need to be aware of and the common pitfalls that should be looked out for when starting in this area.
Diurnal Changes in Transcript and Metabolite Levels during the Iron Deficiency Response of Rice
- Jamie Selby-Pham, Adrian Lutz, Laura T. Moreno-Moyano, B. Boughton, U. Roessner, A. Johnson
- Environmental ScienceRice
- 20 April 2017
This study confirmed that rice shares a similar peak time of Fe deficiency associated induction of DMA secretion compared to other Strategy II plant species but has less prominent daily fluctuations of D MA secretion and revealed metabolic changes associated with the remediation ofFe deficiency and mitigation of damage from resulting stress in rice roots.
Metabolite profiling of wheat (Triticum aestivum L.) phloem exudate
- Lachlan J Palmer, D. Dias, B. Boughton, U. Roessner, R. Graham, J. Stangoulis
- Environmental SciencePlant Methods
- 15 August 2014
This study demonstrates that it is possible to determine metabolite profiles from extremely small volumes ofphloem exudate and that this method can be used to determine variability within the metabolite profile of phloem that has occurred with changes in maturity.