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Multiplex PCR for Enterotoxigenic, Attaching and Effacing, and Shiga Toxin-Producing Escherichia coli Strains from Calves
ABSTRACT A multiplex PCR was developed to identify enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains by amplifying genes encoding K99 and F41 fimbriae,Expand
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Escherichia coli O157:H7 requires intimin for enteropathogenicity in calves.
Enterohemorrhagic Escherichia coli (EHEC) strains require intimin to induce attaching and effacing (A/E) lesions in newborn piglets. Infection of newborn calves with intimin-positive orExpand
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Pathogenicity of Escherichia coli O157:H7 in the intestines of neonatal calves.
Cattle are an important reservoir of Shiga toxin-producing enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains, foodborne pathogens that cause hemorrhagic colitis and hemolytic uremic syndromeExpand
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Bovine Immune Response to Shiga-Toxigenic Escherichia coli O157:H7
ABSTRACT Although cattle develop humoral immune responses to Shiga-toxigenic (Stx+) Escherichia coli O157:H7, infections often result in long-term shedding of these human pathogenic bacteria. TheExpand
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Pathogenesis of Escherichia coli O157:H7 in weaned calves.
Cattle are an important reservoir of Shiga toxin-producing Escherichia coli O157:H7 and other enterohemorrhagic E. coli (EHEC) that cause diarrhea, hemorrhagic colitis, and hemorrhagic uremicExpand
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Prevalences of Some Virulence Genes among Escherichia Coli Isolates from Swine Presented to a Diagnostic Laboratory in Iowa
1. Anver MR, Park JS, Rush HG: 1976, Dermatophilus in the marble lizard (Calotes mystaceus). Lab Anim Sci 26:817–823. 2. Biberstein EL: 1990, The skin as a microbial habitat: bacterial skinExpand
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Design and Evaluation of a Multiplex Polymerase Chain Reaction Assay for the Simultaneous Identification of Genes for Nine Different Virulence Factors Associated with Escherichia Coli that Cause
  • T. Casey, B. Bosworth
  • Biology, Medicine
  • Journal of veterinary diagnostic investigation…
  • 1 January 2009
A multiplex polymerase chain reaction (mPCR) assay was developed for detection and characterization of pathogenic Escherichia coli that cause diarrhea and edema disease in swine. The mPCR assay wasExpand
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Effect of Dietary Stress on Fecal Shedding ofEscherichia coli O157:H7 in Calves
ABSTRACT Two groups of calves were subjected to dietary stress by withholding of food beginning 1 or 14 days after inoculation with 1010 CFU of Escherichia coli O157:H7. Following treatment, neitherExpand
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Expression of Heat-Stable Enterotoxin STb by Adherent Escherichia coli Is Not Sufficient To Cause Severe Diarrhea in Neonatal Pigs
ABSTRACT The role of Escherichia coli heat-stable enterotoxin B (STb) in neonatal porcine diarrhea caused by enterotoxigenic E. coli was examined by comparing adherent isogenic strains with orExpand
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Early Attachment Sites for Shiga-Toxigenic Escherichia coli O157:H7 in Experimentally Inoculated Weaned Calves
ABSTRACT Weaned 3- to 4-month-old calves were fasted for 48 h, inoculated with 1010 CFU of Shiga toxin-positive Escherichia coli (STEC) O157:H7 strain 86-24 (STEC O157) or STEC O91:H21 strain B2F1Expand
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