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Phenotype microarrays for high-throughput phenotypic testing and assay of gene function.
The bacterium Escherichia coli is used as a model cellular system to test and validate a new technology called Phenotype MicroArrays (PMs). PM technology is a high-throughput technology for… Expand
Complete analysis of cellular nucleotides by two-dimensional thin layer chromatography.
We describe methods for the complete analysis of cellular nucleotides from as few as 10(6) 32Pi-labeled cells in a simple 2-day experiment. Nucleotides are extracted with acid, neutralized, and… Expand
Global phenotypic characterization of bacteria
- B. Bochner
- Medicine, Biology
- FEMS microbiology reviews
- 1 December 2008
The measure of the quality of a systems biology model is how well it can reproduce and predict the behaviors of a biological system such as a microbial cell. In recent years, these models have been… Expand
Phenotype microarray analysis of Escherichia coli K-12 mutants with deletions of all two-component systems.
Two-component systems are the most common mechanism of transmembrane signal transduction in bacteria. A typical system consists of a histidine kinase and a partner response regulator. The histidine… Expand
Positive selection for loss of tetracycline resistance.
A simple technique has been devised that allows direct plate selection of tetracycline-sensitive clones from a predominantly tetracycline-resistant population. The technique is especially useful in… Expand
Generalized indicator plate for genetic, metabolic, and taxonomic studies with microorganisms.
We have developed an indicator plate that works well for diverse types of substrates and microorganisms. The plates are inexpensive and easy to prepare. The essential components are agar, buffer,… Expand
Innovations: New technologies to assess genotype–phenotype relationships
- B. Bochner
- Biology, Medicine
- Nature Reviews Genetics
- 1 April 2003
The accelerating pace of the discovery of genes has far surpassed our capabilities to understand their biological function — in other words, the phenotypes they engender. We need efficient and… Expand
Sleuthing out bacterial identities
- B. Bochner
- Chemistry, Medicine
- 11 May 1989
Global phenotype analyses are now possible using a versatile redox chemistry that is readily automated with computerization and microplate technology.
Decreased tryptophan metabolism in patients with autism spectrum disorders
BackgroundAutism spectrum disorders (ASDs) are relatively common neurodevelopmental conditions whose biological basis has been incompletely determined. Several biochemical markers have been… Expand
ZTP (5-amino 4-imidazole carboxamide riboside 5′-triphosphate): A proposed alarmone for 10-formyl-tetrahydrofolate deficiency
Starvation of Salmonella typhimurium for folate in six different ways triggers the intracellular accumulation of a novel ribotide, 5-amino 4-imidazole carboxamide riboside 5'-triphosphate (ZTP).… Expand