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A model for RNA editing in kinetoplastid mitochondria: RNA molecules transcribed from maxicircle DNA provide the edited information
A model is presented in which a partial hybrid formed between the gRNA and preedited mRNA is substrate for multiple cycles of cleavage, addition or deletion of uridylates, and religation, eventually resulting in a complete hybrid between thegRNA and the mature edited mRNA. Expand
Guide RNAs in kinetoplastid mitochondria have a nonencoded 3′ oligo(U) tail involved in recognition of the preedited region
Primary structure calculations indicate a functional role of the 3' oligo(U) tail in stabilizing the initial hybrid formed between the gRNA and the preedited mRNA, and allowed the identification of potential mRNA recognition sites for an editing complex. Expand
Chimeric gRNA-mRNA molecules with oligo(U) tails covalently linked at sites of RNA editing suggest that U addition occurs by transesterification
It is proposed that chimeric molecules are intermediates in the editing process and that successive transesterifications result in the transfer of uridine residues from the gRNA 3' oligo(U) tail to an editing site, with the number ofUridine residues determined by base pairing with adenine and guanine "guide" nucleotides in the g RNA. Expand
The use of transgenic Trypanosoma brucei to identify compounds inducing the differentiation of bloodstream forms to procyclic forms.
The expression of procyclins is the earliest known marker of differentiation of bloodstream forms of Trypanosoma brucei to procyclic forms. We have generated transgenic bloodstream and procyclicExpand
Generation of unexpected editing patterns in Leishmania tarentolae mitochondrial mRNAs: Misediting produced by misguiding
We have analyzed the generation of unexpected patterns of RNA editing, i.e., those not following a strict 3' to 5' progression, which occur in junction regions between fully edited and preeditedExpand
Formation of guide RNA/messenger RNA chimeric molecules in vitro, the initial step of RNA editing, is dependent on an anchor sequence.
  • B. Blum, L. Simpson
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 15 December 1992
In vitro-synthesized chimeras present in steady-state kinetoplast RNA showed no editing of downstream editing sites and may accurately represent the initial step in RNA editing. Expand
Tubulin mRNAs of Trypanosoma brucei.
The tubulin genes of Trypanosoma brucei are located in a single, tightly packed cluster of ten tandemly arranged alternating alpha and beta-genes, which can be assumed to be the locus of tubulin gene expression. Expand
Antimycin A resistance in a mutant Leishmania tarentolae strain is correlated to a point mutation in the mitochondrial apocytochrome b gene
This finding provides direct evidence that edited RNAs are translated in kinetoplastid mitochondria, and a defined mutation conferring drug resistance to a mitochondrial gene product can be exploited for the development of mitochondrial transfection systems for trypanosomatids. Expand
Localization and DNA sequence around the initiation site of ribosomal RNA transcription in Physarum polycephalum.
This work has used S1 nuclease to map the initiation site of ribosomal RNA transcription in the acellular slime mold Physarum polycephalum, and the sequence of 1011 nucleotides surrounding the start site is determined. Expand
Processing in the external transcribed spacer of ribosomal RNA from Physarum polycephalum.
During all interphase stages newly initiated transcripts of rDNA and products of the first processing step are present in similar amounts, indicating control of initiation and not of maturation as being the main regulatory step for the accumulation of mature rRNAs. Expand