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Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations
TLDR
Fluorescent oligonucleotide hybridization probes were used to label bacterial cells for analysis by flow cytometry and the intensity of fluorescence was increased additively by the combined use of two or three fluorescent probes complementary to different regions of the same 16S rRNA. Expand
Application of Digital Image Analysis and Flow Cytometry To Enumerate Marine Viruses Stained with SYBR Gold
TLDR
A novel nucleic acid stain, SYBR Gold, was used to stain marine viral particles in various types of samples and yielded bright and stable fluorescent signals that could be detected by a cooled charge-coupled device camera or by flow cytometry. Expand
Reconsidering the relationship between virally induced bacterial mortality and frequency of infected cells
TLDR
A steady-state model was developed to more ngorously define the relationships between FIC, FVIC, and the fraction of mortality from viral lysis (FMVL), and shows that calculations of FMVL from FIC or FVic are very sensitive to changes in the relative length of the latent period (y) and in the assumed proportion ofThe latent period during which viral particles are not recognizable ( E ) . Expand
Ecological Genomics of Marine Roseobacters
TLDR
Comparative genome sequence analysis of three cultured Roseobacter representatives suggests that cellular requirements for nitrogen are largely provided by regenerated ammonium and organic compounds, while typical sources of carbon include amino acids, glyoxylate, and aromatic metabolites. Expand
Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton
TLDR
Targeted metagenomics analysis of uncultured pico-prymnesiophytes sorted by flow cytometry from subtropical North Atlantic waters reveals a composite nuclear-encoded gene repertoire with strong green-lineage affiliations, which contrasts with the evolutionary history indicated by the plastid genome. Expand
Circadian gating of cell division in cyanobacteria growing with average doubling times of less than 24 hours.
TLDR
It is found that populations dividing at rates as rapid as once per 10 h manifest circadian gating of cell division, since phases in which cell division slows or stops recur with a circadian periodicity. Expand
Dynamics of picophytoplankton, ultraphytoplankton and bacteria in the central equatorial Pacific
TLDR
Changes in the fluorescence and light scatter properties of individual Prochlorococcus cells were observed during the passage of a tropical instability wave during TS-II, and are hypothesized to reflect a physiological response among these cells to that event. Expand
Application of dilution experiments for measuring growth and mortality rates among Prochlorococcus and Synechococcus populations in oligotrophic environments
TLDR
Dilution experiments were used to examine growth and grazing mortality rates among Prochlorococcus and Synechococcus populations in the Sargasso Sea and California Current and it is hypothesized that improved food quality in nutri- ent-replete picoplankton cells may be responsible for these changes. Expand
SEXUALITY AND CYST FORMATION IN THE DINOFLAGELLATE GONYAULAX TAMARENSIS: CYST YIELD IN BATCH CULTURES 1
TLDR
Encystment of the toxic dinoflagellate Gonyaulax tamarensis Lebour (var. excavata) was monitored in batch cultures exposed to a variety of nutritional and environmental treatments, and cyst formation was negligible in nutrient‐replete medium. Expand
Cell Cycle Regulation in Marine Synechococcus sp. Strains
The cell cycle behavior of four marine strains of the unicellular cyanobacterium Synechococcus sp. was analyzed by examining the DNA frequency distributions of exponentially growing and dark-blockedExpand
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