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PhaF, a Polyhydroxyalkanoate-Granule-Associated Protein of Pseudomonas oleovorans GPo1 Involved in the Regulatory Expression System for pha Genes
This work has demonstrated that the phaC1 gene is expressed efficiently in the presence of octanoic acid while its expression is repressed when glucose or citrate is used as the carbon source, and that both PhaF and PhaI proteins are bound to PHA granules produced by P. oleovorans. Expand
Metabolic response of Pseudomonas putida during redox biocatalysis in the presence of a second octanol phase
This study shows that solvent‐tolerant P.’putida have the remarkable ability to compensate for high energy demands by boosting their energy metabolism to levels up to an order of magnitude higher than those observed during unlimited growth. Expand
Metabolic capacity estimation of Escherichia coli as a platform for redox biocatalysis: constraint‐based modeling and experimental verification
Quantitative comparison of computationally predicted NADH regeneration and experimental epoxidation rates indicated that the achievable biocatalytic activity is determined by metabolic and enzymatic limitations including non‐optimal flux distributions, high maintenance energy demands, energy spilling, byproduct formation, and uncoupling. Expand
Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes
Alkane-assimilating yeast Y. lipolytica, coupled with the described expression strategies, demonstrated its high potential for biotransformations of hydrophobic substrates in two-liquid biphasic systems. Expand
Heme-iron oxygenases: powerful industrial biocatalysts?
Are cytochrome P450 enzymes powerful industrial biocatalysts? Next to market demands, well-defined enzyme functionalities and process parameters allow generalizations on the basis of process windows.Expand
The polyhydroxyalkanoate metabolism controls carbon and energy spillage in Pseudomonas putida.
It is demonstrated that the PHA metabolism plays a critical role in synchronizing global metabolism to availability of resources in PHA-producing microorganisms. Expand
Engineering the productivity of recombinant Escherichia coli for limonene formation from glycerol in minimal media
The two‐liquid phase fed‐batch setup led to the highest monoterpene concentration obtained with a recombinant microbial biocatalyst to date, and the growth‐dependent production of (S)‐limonene was shown for the first time in minimal media. Expand
The efficiency of recombinant Escherichia coli as biocatalyst for stereospecific epoxidation
The results point to a product toxicity‐induced biological energy shortage reducing the biocatalyst activity under process conditions, which represents the highest productivity reported for oxygenase‐based whole‐cellBiocatalysis involving toxic products. Expand
Systems biotechnology – Rational whole‐cell biocatalyst and bioprocess design
Microbial biocatalysis has emerged to a standard technology in the food, feed, pharmaceutical, and fine chemical industries. Since microorganisms are optimized by nature to maximize survival andExpand
Enzyme-mediated oxidations for the chemist
Biocatalysis is an enabling technology adding to organic oxidation chemistry. Especially the high selectivity of enzymatic oxidation coevally operating under mild conditions and not necessitatingExpand