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Long-term growth of lymphokine-activated killer (LAK) cells: role of anti-CD3, beta-IL 1, interferon-gamma and -beta.
These approaches may enable the in vitro generation from individual donors of much greater numbers of LAK cells for adoptive immunotherapy than can now be obtained with the 3 to 5 day in vitro culture systems. Expand
Immune responses in vitro. I. Culture conditions for antibody synthesis.
The addition of mercaptoethanol to the culture medium and the optimization of other components has substantially improved the response of mouse spleen cells to in vitro immunization with heterologous RBC and may facilitate the study of the genetic control of antibody synthesis. Expand
Cell Mediated Immunity: Separation of Cells Involved in Recognitive and Destructive Phases
There appears to be a dichotomy both for genetic control of, and cell populations involved in, the recognitive and destructive phases of cell mediated immunity. Expand
Lymphocyte reactivity in vitro. II. Soluble reconstituting factor permitting response of purified lymphocyte.
Evidence is presented which suggests that the role of the added cell population may be an essential one and the existence of such a factor (conditioned medium reconstituting factor—CMRF) may cause us to reevaluate some previous roles suggested for the macrophage in immunological reactions. Expand
Antigenic Requirements for Triggering of Cytotoxic T Lymphocytes
The cotnplexity of T lymphocyte subclasses that respond to alloantigens can be analyzed from at least two perspectives: first, by identification and separation of the different subclasses on theExpand
Lymphocyte reactivity in vitro. I. Cellular reconstitution of purified lymphocyte response.
The data obtained are consistent with an essential role for the added cells, which can be either isogeneic or allogeneic to the responding lymphocytes, and the degree of reconstitution is variable but depends to some extent on the number of added cells. Expand
The cell-mediated lympholytic capability of mouse spleen cells stimulated in mixed lymphocyte culture is related to the major histocompatibility complex genotype on target lymphocytes and serologically defined regions (H-2K and H-2D) are identical within each combination. Expand
Sequence polymorphism of HLA DRβ1 alleles relating to T-cell-recognized determinants
Comparisons of sequences of three HLA DRβ cDNA clones obtained from cells that all express the same serologically defined determinants but differ in terms of T-cell-recognized specificities indicate that very few nucleotides differ between what are almost certainly alleles of the DRβ1 locus. Expand
A “Minimal Signal‐Stepwise Activation” Analysis of Functional Maturation of T Lymphocytes
It is suggested that incompatability for HLA was the basis for reactivity in MLC, and reference to work that might not be otherwise discussed is included to ensure the field will be well represented. Expand