Mesenchymal stem cell durotaxis depends on substrate stiffness gradient strength.
- L. Vincent, Yu Suk Choi, B. Alonso-Latorre, J. C. del Álamo, A. Engler
- BiologyBiotechnology Journal
- 1 April 2013
The data suggest that a functional actin cytoskeleton is required for migration whereas microtubules are required for directed migration, and imply that, in vivo, MSCs may preferentially accumulate in regions of high elastic modulus and make a greater contribution to tissue repairs in these locations.
Roles of cell confluency and fluid shear in 3-dimensional intracellular forces in endothelial cells
- S. Hur, J. C. del Álamo, S. Chien
- Biology, EngineeringProceedings of the National Academy of Sciences
- 4 June 2012
Differences in the directionality and magnitude of intracellular tensions may modulate translation and transcription of ECs under different flow patterns, thus affecting their susceptibility for atherogenesis.
Spatio-temporal analysis of eukaryotic cell motility by improved force cytometry
- J. C. del Álamo, R. Meili, J. Lasheras
- BiologyProceedings of the National Academy of Sciences
- 14 August 2007
An improved force cytometry method is presented and it is found that the mean velocity of migration v and the period of the strain energy T cycle are related through a hyperbolic law v = L/T, where L is a constant step length that remains unchanged in mutants with adhesion or contraction defects.
Three-Dimensional Quantification of Cellular Traction Forces and Mechanosensing of Thin Substrata by Fourier Traction Force Microscopy
- J. C. del Álamo, R. Meili, J. Lasheras
- EngineeringPLoS ONE
- 18 June 2013
The cell's mechanosensing of substratum thickness by 3D traction stresses is considered, finding that, when cells adhere on thin substrata, their out-of- plane traction stresses can reach four times deeper into the substratum than their in-plane traction stresses.
Myosin II Is Essential for the Spatiotemporal Organization of Traction Forces during Cell Motility
- R. Meili, B. Alonso-Latorre, J. C. del Álamo, R. Firtel, J. Lasheras
- BiologyMolecular Biology of the Cell
- 1 February 2010
It is proposed that the motor and actin-crosslinking functions of MyoII differentially control the temporal and spatial distribution of the traction forces, and establish mechanistic relationships between these distributions, enabling cells to move.
Multiple actin binding domains of Ena/VASP proteins determine actin network stiffening
- B. Gentry, Stef Meulen, Philippe Noguera, B. Alonso-Latorre, J. Plastino, G. Koenderink
- BiologyEuropean Biophysics Journal
- 29 September 2012
It is proposed that, in the cell, VASP crosslinking confers only moderate increases in linear network elasticity, and unlike other crosslinkers, V ASP’s network stiffening activity may be tuned by the local concentration of monomeric actin.
The SCAR/WAVE complex is necessary for proper regulation of traction stresses during amoeboid motility
- E. Bastounis, R. Meili, B. Alonso-Latorre, J. C. del Álamo, J. Lasheras, R. Firtel
- BiologyMolecular Biology of the Cell
- 1 November 2011
A combination of traction force and F-actin measurements shows that cells lacking either of the SCAR/WAVE complex proteins SCAR and PIR121 exhibit an altered cell motility cycle and spatiotemporal…
Force and shape coordination in amoeboid cell motility
- B. Alonso-Latorre
- Biology
- 2010
This analysis has elucidated the role that protein myosin II plays in enhancing the kinetics of the four stages of the cycle and in controlling the spatial distribution of the traction forces regulating that process.
An Oscillatory Contractile Pole-Force Component Dominates the Traction Forces Exerted by Migrating Amoeboid Cells
- B. Alonso-Latorre, J. C. del Álamo, R. Meili, R. Firtel, J. Lasheras
- BiologyCellular and Molecular Bioengineering
- 29 June 2011
By compressing the mechanics of chemotaxing cells into a reduced set of temporally-resolved degrees of freedom, the present study may contribute to refined models of cell migration that incorporate cell-substrate interactions.
Distribution of traction forces associated with shape changes during amoeboid cell migration
- B. Alonso-Latorre, R. Meili, E. Bastounis, J. C. del Álamo, R. Firtel, J. Lasheras
- BiologyAnnual International Conference of the IEEE…
- 13 November 2009
The results indicate that the mechanical cycle of traction stresses and cell shape changes remains remarkably similar for all cell lines but is slowed down when myosin function is lost, probably due to a reduced control on the spatial organization of the traction stresses.
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