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Adipocytes were isolated from biopsies of the subcutaneous adipose tissue of dairy cows before and after parturition. The diameter of the cells was measured and their lipogenic and lipolytic capacities were assessed by measurement of 14C-glucose incorporation and glycerol release. The concentration of non-esterified fatty acids (NEFA) in plasma at the time(More)
The degree of fatty infiltration of the liver was estimated in two herds of dairy cows using biochemical and stereological methods. Estimation of hepatic triglyceride content biochemically or of fractional volume of fat in hepatocytes stereologically are both reliable methods of assessing fatty infiltration of the liver in the dairy cow. Estimation of(More)
The metabolic capacity of bovine adipocytes was studied using isolated cells from subcutaneous adipose tissue of four dairy cows before and after calving. The lipogenic capacity was assessed by measuring the incorporation of 14C-glucose and 14C-acetate into lipid. In the cells taken from the cows before calving, 14C-acetate was incorporated into lipid at a(More)
Solutions containing sodium propionate or sodium acetate were infused into a mesenteric vein of eight steers in order to examine the effect of increasing the entry rate of these metabolites on feed intake. Infusion of propionate inhibited feed intake to varying degrees, but acetate infused at equivalent rates had no effect. Rate of entry of propionate into(More)
Adipose tissue samples were taken from five dairy cows in early lactation. Various external and internal sites were chosen, and after isolation of the adipocytes, their size and lipolytic capacity were measured. It was found that cells from all the sites showed high lipolytic activity, although there was a four-fold difference between cells from perirenal(More)
To investigate diabetes resistance to T cell-mediated disease transfer, we administered islet-specific T cell clones to the F1 progeny of nonobese diabetic (NOD) mice that were crossed with various nondiabetes-prone inbred mouse strains. We investigated four diabetogenic CD4+ T cell clones and all induced insulitis and full development of diabetes in (SWR x(More)
An enzymatic method for the measurement of non-esterified fatty acid (NEFA) concentrations in blood was applied to samples from dairy cows. The method was carried out on a discrete analyser and showed considerable savings over other methods, particularly in time. The precision of the method was very high and the accuracy was good within normal concentration(More)
The lipids of two cell types (primary pig kidney and secondary adult pig thyroid) and those of transmissible gastroenteritis virus (TGEV) grown in these cells were studied using 14C-palmitic acid. Differences were demonstrated between the incorporation of isotopically labelled lipid precursors in the two cell types and it was found that the phospholipid and(More)
B lymphocytes with receptors specific for the hapten fluorescein (FLU) were prepared from the spleens of mice of various ages. For most experiments, a one-step fractionation procedure based on the adherence of FLU-specific cells to FLU-gelatin was used. For some experiments, a subset of higher FLU-binding capacity was prepared from the FLU-gelatin binding(More)