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High-speed detection of blood-borne hepatitis C virus RNA by single-tube real-time fluorescence reverse transcription-PCR with the LightCycler.
Hepatitis C virus (HCV) has been identified as the agent responsible for the vast majority of cases of posttransfusion non-A, non-B hepatitis. Although generally asymptomatic, ∼85% of the infectionsExpand
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Two-round rapid-cycle RT-PCR in single closed capillaries increases the sensitivity of HCV RNA detection and avoids amplicon carry-over.
BACKGROUND For the detection of hepatitis C virus (HCV) specific nucleic acids the polymerase chain reaction (PCR) is widely used. Rapid-cycle PCR is performed in glass capillaries with theExpand
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