B. N. Postrigan’

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In the process of obtaining transgenic plants suitable for phytoremediation of heavy metal-contaminated soils, a synthetic pseudophytochelatin gene coding for a phytochelatin analog Met(GluCys)6Gly was de novo designed and cloned. Contrary to natural enzymatically synthesized phytochelatins, this peptide can be made by a template synthesis. A construct(More)
Transgenic tobacco plants expressing the fragments of the ARGOS and NtEXPA4 genes in antisense orientation have been created. Eleven lines of transgenic plants were investigated and five of them were characterized by a decrease in the sizes of the leaves and flowers as compared to control. Stem sizes decreased when only the NtEXPA4 gene fragment was used.(More)
The effect of cadmium acetate (200 and 400 μM Cd(CH3COO)2) on the transcriptional promoter activity of rice (Oryza sativa L.) phytochelatin synthase gene 900 bp in size was studied. The reporter gene of glucuronidase was placed under the control of this promoter in transgenic tobacco (Nicotiana tabacum L.) plants. For determining boundaries of this gene(More)
The AINTEGUMENTA gene encodes a transcription factor involved in the regulation of growth of both reproductive and vegetative plant organs. Two conserved regions of this gene were amplified and cloned in antisense orientation. The produced genetic constructs were used to obtain transgenic tobacco plants with the AINTEGUMENTA gene expression downregulated.(More)
Screening of nodule bacteria (rhizobia) forming symbiotic relationships with legumes has been performed in order to isolate strains resistant to cadmium ions in a wide range of concentrations (6–132 mg/kg). The effect of cadmium salts (6, 12, 24 mg/kg) on the legume-rhizobium symbiosis of the pea Pisum sativum L. with Rhizobium leguminosarum and of the(More)
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