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1. Single cultured pig aortic smooth muscle cells were studied using fura-2 and dual excitation wavelength microfluometry. 2. Extracellular ATP in micromolar concentrations induced a transient increase of [Ca2+]i due to Ca2+ release from internal stores. In the same concentration range application of ATP resulted in an increase of intracellular inositol(More)
The effects of electrical field stimulation on [Ca2+]i in the A7r5 vascular smooth muscle cell line have been monitored with the Ca(2+)-sensitive dye fura-2. The experimental set-up allowed high-temporal resolution of the [Ca2+]i-measurements and fast application of test solutions. Electrical field stimulation of A7r5 cells in the confluent growth state(More)
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