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Marked alterations in astrocyte function are a universal response to disease or injury in the central nervous system. Affected astrocytes develop characteristic morphological changes known as "reactive astrogliosis", characterized by increased expression of the intermediate filament proteins, glial fibrillary acidic protein and vimentin. Reactive astrocytes(More)
Nucleofection is a powerful non-viral transfection technique that can deliver plasmid DNA with high efficiency to cells that are traditionally difficult to transfect. In this study, we demonstrate that nucleofection of astrocytes grown in primary cell culture resulted in 76 ± 9% transfected cells and low cytotoxicity. However, the nucleofected astrocytes(More)
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