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During the development of the vertebrate nervous system, many neurons depend for survival on interactions with their target cells. Specific proteins are thought to be released by the target cells and to play an essential role in these interactions. So far, only one such protein, nerve growth factor, has been fully characterized. This has been possible(More)
The mRNAs of nerve growth factor (NGF) and brain derived neurotrophic factor (BDNF) exhibit a similar, though not identical, regional and cellular distribution in the rodent brain. In situ hybridization experiments have shown that BDNF, like NGF, is predominantly expressed by neurons. The neuronal localization of the mRNAs of these two neurotrophic(More)
Lesion of the sciatic nerve caused a rapid increase in c-fos and c-jun mRNA that was followed about 2 hr later by an increase in nerve growth factor (NGF) mRNA. To evaluate whether the initial increase in c-fos mRNA is causally related to the subsequent increase in NGF mRNA, we performed experiments with fibroblasts of transgenic mice carrying an exogenous(More)
The lymphokine interleukin 1 (IL-1) rapidly increases the levels of mRNA encoding nerve growth factor (NGF) in primary cultures of fibroblasts isolated from adult rat sciatic nerves. The effect of IL-1 on NGF-mRNA was not inhibited by cycloheximide, which itself increased the NGF-mRNA. From the blocking ability of actinomycin D, it was deduced that the IL-1(More)
Transforming growth factor-beta 1 (TGF-beta 1) markedly increased the mRNA encoding nerve growth factor (NGF) in cultured rat astrocytes in a time- and concentration-dependent manner. The maximal effect of TGF-beta 1 (a 50-fold increase in NGF-mRNA) was reached after 24 h incubation. The TGF-beta-mediated increase in NGF-mRNA results from enhanced(More)
Both nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) exert neurotrophic actions on the cholinergic neurons of the basal forebrain nuclei. These neurotrophic factors are synthesized by hippocampal and cortical neurons that are located in the projection field of the basal forebrain cholinergic neurons. Both in vivo and in vitro the(More)
Many theories of memory postulate that processing of information outlasts the learning situation and involves several different physiological substrates. If such physiologically distinct mechanisms or stages of memory do in fact exist, they should be differentially affected by particular experimental manipulations. Accordingly, a selective improvement of(More)
BACKGROUND Clinical evidence indicates that vascular endothelial cell (EC) dysfunction occurs early after transplantation (Tx) and initiates chronic graft vasculopathy. This study explored this phenomenon in rat aorta Tx using the stringent Dark Agouti (DA)-to-Lewis (LEW) and the weak Fischer 344 (F344)-to-LEW strain combinations. METHODS Donor abdominal(More)