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In diabetes mellitus, hyperglycemia is often associated with elevated levels of glucagon in the blood. This suggests that glucagon is a contributing factor in the metabolic abnormalities of diabetes mellitus. A glucagon-receptor antagonist would provide direct evidence for glucagon's role in diabetes mellitus. On the basis of careful consideration of(More)
The interaction of the opioid enkephalins and endorphins with lipid bilayers is largely unknown. Such interactions might, however, be important for understanding the molecular mechanisms of biological action. We have therefore studied the interaction of several enkephalins and of dynorphin-(1-13)-tridecapeptide (dynorphin1-13) with model membrane systems,(More)
In an effort to obtain highly potent glucagon antagonists, we have investigated glucagon (1) structure-function relationships utilizing the following design principles: (1) structural changes known to lead to partial agonist activities; (2) conformational restrictions; (3) changes in the conformational probabilities of the primary sequence; and (4)(More)
The hyperglycemia and ketosis of diabetes mellitus are generally associated with elevated levels of glucagon in the blood. This suggests that glucagon is a contributing factor in the metabolic abnormalities of diabetes mellitus. A glucagon-receptor antagonist might provide important evidence for glucagons's role in this disease. In this work we describe how(More)
We have used glucagon and nine glucagon analogs to investigate the interactions of these ligands with glucagon-binding sites present on isolated canine hepatocytes. Curves reflecting the inhibition of 125I-labeled glucagon or 125I-labeled analog binding to cells by the 10 peptides spanned, overall, a 10(6)-fold range of hormone concentration, were(More)
Occurrence and growth-promoting effect suggest that epidermal growth factor (EGF) is involved in the maintenance of the gastrointestinal mucosa. The aim of this study was to ascertain whether rats with gastric lesions, induced by cold-water stress, ethanol, or indomethacin, have altered EGF levels in their gastrointestinal tract compared with controls. For(More)
To test our hypothesis that specific interactions of ACTH peptides with model lipid membranes reflect the biological importance of similar interactions on target cells, we investigated the liposome-mediated labeling of ACTH fragments with the extremely hydrophobic photolabel, 3-trifluoromethyl-3-(m-[125I]iodophenyl)diazirine. Correlations were found between(More)
Hydrophobic photolabeling with 3-(trifluoromethyl) -3-(m-[125I]iodophenyl) diazirine ( [125I]TID [Brunner, J., & Semenza , G. (1981) Biochemistry 20, 7174-7182] ) and equilibrium dialysis were used to study hydrophobic and electrostatic interactions between three adrenocorticotropin fragments and liposomes prepared from mixtures of phosphatidylcholine with(More)
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