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OBJECTIVE To investigate the relationship between the gene polymorphism of the endothelial nitric oxide synthase enzyme (eNOSG894T) with pulmonary hypertension of Chinese patient with chronic obstructive pulmonary disease (COPD). METHODS Fifty normal volunteers (29 male, 21 female, mean age 67 +/- 3) and 50 patients with COPD complicated by pulmonary(More)
OBJECTIVE To clone the rpoB gene of M. tuberculosis. METHODS Screening the rpoB gene of M. tuberculosis from M. tuberculosis genomic DNA library by using the rpoB gene conservative region PCR product as a probe. RESULTS 411 bp products were seen after amplification of H37Ra DNA. The cloning and sequencing results indicated that the 411 bp products were(More)
Percutaneous coronary artery revascularization has now evolved to a status where angioplasty and stenting have become routine practice for the treatment of significant obstructive atherosclerotic vascular disease. Angioplasty and stenting have become routine practice for the treatment of significant obstructive atherosclerotic vascular disease. Neointimal(More)
OBJECTIVE To evaluate the applicability of detection of rpoB gene mutation in M. tuberculosis susceptibility testing. METHODS 87 M. tuberculosis isolates and 22 sputum specimens from patients with active pulmonary tuberculosis were detected by PCR-SSCP. RESULTS The sensitivity of PCR for rpoB gene amplification was 100 pg DNA and 5000 organisms. The(More)
OBJECTIVE To provide effective tools for identification and characterization of M. tuberculosis genes/antigenes and to evaluate their roles in diagnosis, vaccination, drug resistance, and pathogenesis. METHODS M. tuberculosis genomic DNA obtained from H 37 Ra strain was partially digested with DNase I. The DNA fragments ranging from 4-8 kb were isolated(More)
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