B C Antonellis

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1. Scanning electron microscopy of minced dogfish, shark, and human lens fiber cell preparations showed that neither 8 M urea or 1% SDS was capable of totally disrupting the fiber cells, but that their membranes were totally disrupted by 1% SDS containing 50 mM dithiothreitol. 2. Fiber cells were purified by sucrose centrifugation and extracted with(More)
The influence of in vitro exposure of dogfish (Mustelus canis) lenses to H2O2 was studied. Concentrations of H2O2 greater than 10(-4)M caused opacities in the outer cortex of the lens within a few hr. Evidence of protein aggregation within the lens and in extracted lens protein solutions was found. Dithiothreitol inhibits aggregation. Little if any(More)
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