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Cells from human endocrine tumors of proposed neural crest origin--five pheochromocytomas, two medullary carcinomas of the thyroid, and two bronchial carcinoids--were grown in monolayer culture. Cells from all nine tumors, including epithelial forms of medullary carcinoma of the thyroid and bronchial carcinoid cells, and epithelial and neuron-like(More)
1. Adult human and gerbil adrenal medullary cells were maintained in dissociated cell culture and studied by micro-electrode penetration. 2. In the best recordings, chromaffin cell transmembrane potentials exceeded -50mV. 3. Chromaffin cells were capable of generating all-or-nothing over-shooting action potentials, similar to those generated by sympathetic(More)
Serial biochemical studies of a rat cortical tissue culture system in which synapses regularly form showed that gamma-aminobutyric acid (GABA) is present in the cultures and increases with their maturation. The tissue GABA concentration in mature cultures is similar to that of adult rat cortex in vivo. The synthetic enzyme, glutamate decarboxylase, also(More)
Cultured tumour cells from two oat cell carcinomas of the lung were demonstrated to generate all-or-nothing, short-duration action potentials similar to those previously reported in neurones, in bronchial carcinoid cells, and in a number of endocrine cells of proven or proposed neural ancestry. This observation provides new evidence to establish oat cell(More)
Rat cortical neurons in 4- to 7-week-old dissociated cell cultures have action potentials (APs) which are tetrodotoxin-sensitive and appear to be generated by conventional sodium mechanisms. Approximately 10% of neurons can also generate APs when sodium channels are blocked. These latter APs are smaller, slower and sensitive to cobalt. When voltage(More)
Several endocrine cells or their neoplastic derivatives generate action potentials similar to those seen in neurones, and in the adrenal chromaffin cell such regenerative potentials depend primarily on a sodium mechanism. Kidokoro has described action potentials in the GH3 rat pituitary cell line which seem to depend on a calcium mechanism. We have(More)
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