Learn More
Bacterial adenosine triphosphate (ATP) was determined in blood culture bottles using bioluminescence. Seventy bottles were inoculated with blood and bacteria. Immediately after inoculation and every 2 h thereafter for the next 10 h, bacterial ATP was measured as well as the number of colony-forming units (CFU) per millilitre of blood culture bottle. The(More)
The amount of adenosine triphosphate (ATP) in human lymphocytes was determined using a technique based on light emission from a bioluminescent reaction with luciferin-luciferase. The amount of ATP changed when cells were incubated in the presence of specific HLA antisera and complement. For determination of intracellular ATP a modified method was applied,(More)
Results of a comparative study for the detection of significant bacteriuria (greater than 10(5)/ml) under routine conditions in 300 urine samples were reported. The urine specimens were obtained from patients of different clinical departments of our university hospital. Only midstream urines were investigated. The results from ATP determination with(More)
The antimicrobial susceptibility ofMycobacterium tuberculosis H37Rv and 17 clinical isolates of the same species was determined using a bioluminescence assay to measure adenosine triphosphate (ATP) produced by bacteria in the broth dilution test performed in Dubos-Tween-albumin broth. The results were compared with those obtained with the standard dilution(More)
A bioluminescence assay for the rapid detection of bacteria in blood culture bottles was developed and the results compared with the findings of microscopic examination. For this purpose 50 culture bottles supplemented with blood were inoculated with bacteria. After 4, 6 and 7 h of incubation at 37 degrees C samples were removed and examined microscopically(More)
  • 1