B A Bedell

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The dramatic activation of serine proteases in nonaqueous media resulting from lyophilization in the presence of KCl is shown to be unrelated to relaxation of potential substrate diffusional limitations. Specifically, lyophilizing subtilisin Carlsberg in the presence of KCl and phosphate buffer in different proportions, ranging from 99% (w/w) enzyme to 1%(More)
The rational control over enzyme-catalyzed regioselectivity has been studied using sucrose acylation by vinyl esters in organic media as a model. Subtilisins BPN' and Carlsberg preferentially acylate at the 1'-hydroxyl of sucrose with some acylation observed at the 6-hydroxyl. The preference for the 1'-hydroxyl is strongly affected by the hydrophobicity of(More)
An experimental study was conducted to examine the relationship between a nutrition education program and nutrition knowledge and eating behaviors of older adults. Twenty-four subjects were randomly assigned to an experimental or a control group. Prior to any intervention, all subjects completed a demographic data sheet, a 25-item True/False questionnaire(More)
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