B. A. Atakhanova

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Great attention is now being paid to elucidating the correlation between the nature of DNA methylation and the functional status of the cell, since DNA methylation is thought to be one of the factors responsible for the regulation of gene activity [1]. Earlier, we studied the effect of thyroid hormones on DNA methylation in rat liver in rive and in vitro(More)
Protein synthesis (in particular, thyroglobulin) is studied in a cell-free system containing polyribosomes of thyroid gland. After the incubation with radioactive label, ribosomes were centrifugated, supernatant was dialyzed against 0.05 M Tris-HCl buffer, pH 7.2 for a night, concentrated by ficol and centrifugated in 5-20% linear sucrose gradient.(More)
Highly purified thyroglobulin mRNA was isolated from human nodal euthyroid goiter. Full length cDNA was synthesized from 33S RNA by using reverse transcriptase in the presence of human placenta ribonuclease inhibitor. DNA complementary to human Tg mRNA was used in liquid hybridization experiments to determine the quantity of Tg mRNA. The amount of Tg mRNA(More)
The heterogeneity and some properties of DNA-methylases isolated from nonmalignant human thyroid formations--nodular and diffuse goiters--have been studied. Isoelectrofocusing of methylase preparations produced 6-7 distinct activity peaks distinguished by pI, activity towards Ca2+ and Mg2+, sensitivity towards dithiothreitol and capacity to methylase(More)
The content of individual thyreoglobulin (TG) polyribosomes in human thyroid gland was studied in healthy persons and in patients with thyroid pathology. The content of TG polyribosomes makes up to 16.2% in normal thyroid tissue and is found to decrease down to 7.8% and 7% under nodal euthyroid goiter and toxic adenoma, respectively. This content is(More)
The effect of thyroid hormones and the methylation inhibitor 5-azacytidine on the level of expression of thyroid hormone-responsive genes (malic enzyme and 6-phosphogluconate dehydrogenase genes) has been studied. DNA-RNA hybridization has shown there is an inverse correlation between the level of DNA methylation and gene expression of malic enzyme and(More)
Thin-layer chromatography was used for fractionation of rat liver nuclear phospholipids after in vivo administration of 14C-sodium acetate. The administration of T3 to thyroidectomized rats caused a sharp increase in the incorporation of the label in all phospholipids of the nuclear fraction. The action of sphingomyelin and sphingomyelase on RNA-polymerase(More)