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A rapid microcentrifuge-based method is described for preparation of Pseudomonas aeruginosa electrocompetent cells with up to 10,000-fold increased transformation efficiencies over existing procedures. This increased efficiency now enables the use of transformation for all applications requiring DNA transfer. These include transfer of chromosomal mutations(More)
Because of Burkholderia pseudomallei's classification as a select agent in the United States, genetic manipulation of this bacterium is strictly regulated. Only a few antibiotic selection markers, including gentamicin, kanamycin, and zeocin, are currently approved for use with this bacterium, but wild-type strains are highly resistant to these antibiotics.(More)
This paper reports the IIT-TUDA participation in the SemEval 2016 shared Task 5 of Aspect Based Sentiment Analysis (ABSA) for sub-task 1. We describe our system incorporating domain dependency graph features, dis-tributional thesaurus and unsupervised lexical induction using an unlabeled external corpus for aspect based sentiment analysis. Overall, we(More)
In this study, we examined the impact of various environmental conditions on the expression of resistance-nodulation-division (RND) efflux pumps and outer membrane (OM) porins, two key determinants of Acinetobacter baumannii's intrinsic resistance, an organism known to cause various multidrug resistant infections in immunocompromised individuals.(More)
Antibiotic resistance of bacterial pathogens is a fast emerging global crisis and an understanding of the underlying resistance mechanisms is paramount for design and development of new therapeutic strategies. Permeability barriers for and active efflux of drug molecules are two resistance mechanisms that have been implicated in various infectious outbreaks(More)
Construction and integration of recombinant mini-Tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe Pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. Using this method, the Burkholderia pseudomallei bpeEF-oprC operon was shown to encode a(More)
Serratia marcescens is an important nosocomial agent known for causing various infections in immunocompromised individuals. Resistance of this organism to a broad spectrum of antibiotics makes the treatment of infections very difficult. This study was undertaken to identify multidrug resistance efflux pumps in S. marcescens. Three mutant strains of S.(More)
OBJECTIVES To analyse the expression of resistance-nodulation-division (RND) efflux pumps and outer membrane porins during various growth phases of Acinetobacter baumannii ATCC 19606. METHODS Expression of five different RND pump-encoding genes (adeB, adeG, adeJ, AciBau_2436 and AciBau_2746) and two outer membrane porin-encoding genes (carO and oprD) was(More)
BACKGROUND Bacterial pathogens belonging to the genus Acinetobacter cause serious infections in immunocompromised individuals that are very difficult to treat due to their extremely high resistance to many antibiotics. OBJECTIVE To investigate the role of resistance-nodulation-division (RND) pumps and porins in the antibiotic resistance of Acinetobacter(More)
AdeFGH is a multidrug resistance efflux pump of Acinetobacter baumannii belonging to the resistance-nodulation-cell division (RND) family. Analysis of expression of this pump established it as the most prevalent RND pump in clinical isolates of A. baumannii from Canadian hospitals. In this study, the activity of the efflux pump inhibitor (EPI)(More)