Ayumi Yasuoka

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A genomic DNA encoding a subtype adrenoceptor (AR) was cloned from Medaka fish, Oryzias latipes, using an oligonucleotide probe corresponding to the consensus sequence of mammalian alpha-AR and beta-AR. The gene spans at least 9kbp, and the coding region consists of two exons split by an intron of 7.2 kbp located at the same position as those of mammalian(More)
We cloned and characterized two subfamilies of olfatory receptor (OR) genes from medaka fish (Oryzias latipes). Southern blot analysis showed that each of the two subfamilies, designated as subfamilies Y and E, consists of about five members, as usually observed for other vertebrate ORs. Analyses of the genomic clones encoding these members revealed that(More)
Four types of cDNA clones encoding tyrosine kinases highly homologous to mammalian fibroblast growth factor receptors (FGF-R) were isolated from Medaka fish (Oryzias latipes) by the reverse transcription-polymerase chain reaction. Comparison of the four deduced amino acid sequences with four known mammalian FGF-Rs indicated that four FGF-R species(More)
Main olfactory receptor genes were isolated from a seawater fish, Fugu rubripes (pufferfish), and characterized. Two subfamilies of genes encoding seven transmembrane receptors were identified; one consists of five or more members, termed FOR1-1 to 5 of FOR1 subfamily, and the other appears to be a single copy gene, termed the FOR2 subfamily. FOR1 members(More)
In vertebrates, the taste system provides information used in the regulation of food ingestion. In mammals, each cell group within the taste buds expresses either the T1R or the T2R taste receptor for preference-aversion discrimination. However, no such information is available regarding fish. We developed a novel system for quantitatively assaying taste(More)
The distribution of fibroblast growth factor (FGF) and of the mRNA for its receptor encoded by MFR1 were examined in the testis of the medaka, Oryzias latipes. FGF was detected in the region of somatic cells, especially in Sertoli cells in the matured testis by immunostaining. The staining was also detected in some of the type A spermatogonia. The(More)
Previous studies have identified many cDNA species that encode a variety of G protein alpha-subunits occurring in taste buds. These include the cDNA encoding a taste-bud-specific Galpha, gustducin (G(gust)). Here we carried out comprehensive analyses of Galpha species that occur in the taste buds of rat circumvallate papillae and also in their single cells(More)
Fish perceive water-soluble chemicals at the taste buds that are distributed on oropharyngeal and trunk epithelia. Recent progress in molecular analyses has revealed that teleosts and mammals share pivotal signaling components to transduce taste stimuli. The fish orthologs of taste receptors, fT1R and fT2R, show mutually exclusive expression in taste buds,(More)
Several recent papers have reported the difficulties in expressing olfactory receptor proteins (ORs) in heterologous systems, and proposed that some sequences in ORs have negative effects on their efficient expression. To obtain an efficient expression system of ORs, we modified N-terminal sequences of ORs through the addition of exogenous sequences. Three(More)
A genomic DNA fragment encoding a G protein-coupled seven-transmembrane receptor was isolated from Medaka fish, Oryzias latipes. The encoded protein is similar in sequence to other receptors including catecholamine, histamine and serotonin receptors. However, the similarity is much lower than those among members of these receptor subfamilies, thus(More)