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A primary advantage of label-free detection methods over fluorescent measurements is its quantitative detection capability, since an absolute measure of adsorbed material facilitates kinetic characterization of biomolecular interactions. Interferometric techniques relate the optical phase to biomolecular layer density on the surface, but the conversion(More)
A novel method is proposed for direct detection of DNA hybridization on microarrays. Optical interferometry is used for label-free sensing of biomolecular accumulation on glass surfaces, enabling dynamic detection of interactions. Capabilities of the presented method are demonstrated by high-throughput sensing of solid-phase hybridization of(More)
We demonstrate an accurate, quantitative, and label-free optical technology for high-throughput studies of receptor-ligand interactions, and apply it to TATA binding protein (TBP) interactions with oligonucleotides. We present a simple method to prepare single-stranded and double-stranded DNA microarrays with comparable surface density, ensuring an accurate(More)
The conformation of a three-dimensional polymeric coating (copoly(DMA-NAS-MAPS)) and immobilization and hybridization of DNA strands on the polymer coated surface are investigated. A conformational change, specifically the swelling of the surface adsorbed polymer upon hydration, is quantified in conjunction with the application of this polymer coating for(More)
A platform for in situ and real-time measurement of protein-induced conformational changes in dsDNA is presented. We combine electrical orientation of surface-bound dsDNA probes with an optical technique to measure the kinetics of DNA conformational changes. The sequence-specific Escherichia coli integration host factor is utilized to demonstrate(More)
The resonant cavity imaging biosensor (RCIB) is an optical technique for detecting molecular binding interactions label free at many locations in parallel that employs an optical resonant cavity for high sensitivity. Near-infrared light centered at 1512.5 nm couples resonantly through a Fabry-Perot cavity constructed from dielectric reflectors (Si/SiO(2)),(More)
Landslide is one of the most costly and damaging natural hazards of the world, and has direct and indirect influence on a number of human activities. The term landslide includes a wide range of mass movement, such as rock falls, deep failure of slopes and shallow debris flows. Aerial imagery is a very powerful tool for landslide monitoring because it offers(More)
Optical resonators designed for biological sensing and imaging are demonstrated to yield sub-nanometer position accuracy in DNA conformation, high-sensitivity in ring-resonators for biosensing, and massively parallel, non-labeled detection in a Resonant Cavity Imaging Biosensor. Resonance is an enabling property of a myriad of optical devices such as(More)
A platform for in situ and real-time measurement of protein induced conformational changes in dsDNA is presented. We demonstrate protein induced bending upon sequence specific binding of Integration Host Factor (IHF) to dsDNA probes. I. INTRODUCTION Several platforms were recently developed to study protein-DNA interactions. The most popular assays,(More)
We present biological imaging and sensing methods based on optical resonance and interference. In fluorescence microscopy, our nanoscale imaging capability sheds light onto conformational changes of DNA, DNA-protein complexes and polymer coatings on a solid surface. Interference measurements on a layered substrate yield a label-free sensing platform for(More)
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