Learn More
Mustard oil (MO) is a plant-derived irritant that has been extensively used in experimental models to induce pain and inflammation. The noxious effects of MO are currently ascribed to specific activation of the cation channel TRPA1 in nociceptive neurons. In contrast to this view, we show here that the capsaicin receptor TRPV1 has a surprisingly large(More)
TRPA1 is a nonselective cation channel activated by a wide variety of noxious chemicals. Intriguingly, several TRPA1 modulators induce a bimodal effect, activating the channel at micromolar concentrations and inhibiting it at higher concentrations. Here we report the bimodal action of cinnamaldehyde (CA) and camphor, which are thus far reported as agonist(More)
Peptide synthesis and purification Peptides used in this study, termed pep1 (LKRLQKLLSKIWNKW), pep2 (FKRWQKLLSKIWWKN) and pep3 (LKRLQKLLSKIWWKN), were synthesized using 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase methods on Rink amide 4-methyl benzhydrylamine resin (Novabiochem) (0.55 mmol/g) and a Liberty microwave peptide synthesizer (CEM Co. Matthews,(More)
We present herein a first proof of concept demonstrating the potential of a protein nanopore-based technique for real-time detection of selected Gram-negative bacteria (Pseudomonas aeruginosa or Escherichia coli) at a concentration of 1.2 × 10(8) cfu/mL. The anionic charge on the bacterial outer membrane promotes the electrophoretically driven migration of(More)
  • 1