Aulis Marttinen

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BACKGROUND & AIMS The gut epithelium in the crypt-villus axis represents a continuous developmental system in which the role of fibroblast-epithelial interactions is obvious. The aim of this study was to establish an in vitro method whereby fibroblast-guided differentiation of crypt-like gut epithelial cells can be studied. METHODS Intestinal epithelial(More)
To determine the effects of early antigen exposure on the maturation of the gastrointestinal mucosal barrier, rat pups were divided into three groups at the age of 14 d. In addition to normal maternal milk, group CM (n = 24) received daily a gavage feed of cow's milk and group PH (n = 20) a whey protein hydrolysate during the experimental feeding period(More)
The in vivo glucose uptake and the levels of two glucose transporter proteins (GLUT1 and GLUT4) were measured in heart and in various types of skeletal muscle from streptozotocin-diabetic rats. Diabetes (12-16 weeks) reduced the in vivo glucose uptake (glucose metabolic index, GMI), and the levels of GLUT1 and GLUT4 in heart by 75%, 60% and 70%,(More)
A bone morphogenetic protein purification method for minor quantities of bone material was developed based on collagenase splitting of bone connective tissue. Our aim was to remove and characterize the osteoinductive protein preparation in native form without using strongly dissociative agents. We started from 80 g of HCl-demineralized reindeer bone(More)
Using type IV collagen as carrier, the expression of bovine bone morphogenetic protein (bBMP) activity and the relation of ectopic bone formation to BMP dosage in the reconstitution were investigated in BALB mice. Visible heterotopic bone was induced by GuHCl-extracted bovine BMP at a minimal dose of 0.5 mg within 21 days after the BMP was covalently bound(More)
In local or metastatic cancer, a prognostic tumour marker could be a valuable tool in the selection of different treatments. In renal cell cancer (RCC) no such markers have been available. We therefore evaluated the association between several pretreatment serum markers, tumour classification and short term survival in RCC patients. Serum samples were(More)
Noncollagenous proteins, including bone morphogenetic protein (BMP), were extracted in 4 mol/l guanidinium hydrochloride (GuHCl) from the pulverized and HCl-demineralized matrix of reindeer, bovine, sheep and porcine bone. To remove water-soluble material, the GuHCl solution was dialyzed against water and water-insoluble material and redissolved in 4 mol/l(More)
Noncollagenous protein material was extracted from HCl-demineralized bovine bone particles in 4 M guanidinium hydrochloride. Water- and citrate buffer-insoluble material was collected, solubilized in 6 M urea and fractionated by preparative isoelectric focusing using a running voltage of 5000 V. The material removed from the area between pH 4.7 and 5.7 of(More)
dans le nouvel os minéralisé. Le degrés d’intégration de calcium45 s’avère être élevé dans les composés de mBMP et de collagène de type IV, ou de BMP seule, mais les niveaux les plus élevés concernant l’activité de la BMP apparaissent dans les souris où sont implantés des composés de dérivés de corail hydroxyapatite et de mBMP, de façon significativement(More)
We identified and purified six human noncollagenous protein molecules that specifically bind to serum IgA from patients with coeliac disease, and which as a combination can act as true antigen to reticulin antibodies. In affinity chromatography, the purified human protein molecules removed antibodies against reticulin and endomysium from serum samples of(More)