Augustus B. Wadsworth

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In my first paper on " ' Studies on Pneumococcus Infection in Animals, I summarized in the introduction the significant results of previous work and then recorded my experimental studies on the action of the pneumococcus on animal tissues and of immune sera on the pneumococcus. The present paper deals with .the action of immune sera on pneumococcus(More)
It has been possible by rapid transfers alone, not only to maintain the virulence for mice of the pneumococcus in artificial media but also to restore a certain degree of virulence to cultures previously rendered non-virulent by less rapid transfers in the same medium. For these results the presence of enriching fluids such as blood or serum is not(More)
Horses immunized to Type I pneumococci developed serum, 0.1 cc. of which protected against 0.5 cc. of a virulent culture, 0.000001 cc. of which killed mice in less than 40 hours. Protective tests of serum from horses immunized to Type II organisms varied, 0.1 cc. protecting, however, in certain instances against 0.1 and 0.01 cc. of virulent homologous(More)
Antigens were prepared from the culture filtrates of tubercle bacilli and by extraction of washed and dried organisms with organic and aqueous solvents and from tissues of organs showing tuberculous lesions. A comparison of these preparations by means of the complement fixation test showed that the aqueous extracts were most active antigenically. The(More)
By precipitation with acetone at 4 degrees C. a refined diphtheria toxoid was obtained as a dry powder, readily soluble in aqueous solutions. The powder itself appears to be stable. When dissolved in half the original volume of physiological salt solution, the toxoid remained stable, in the cold room, for a period of 7 months. Only toxoids should be used(More)
IT HAS BEEN SHOWN THAT THE PNEUMOCOCCUS MULTIPLYING IN THE TISSUES OF THE IMMUNIZED ANIMAL (HORSE) BECOMES ATTENUATED: loses, in varying degrees, its virulence, capacity of capsule formation, susceptibility to phagocytosis, and type specificity. The antigenic activity as an immunizing agent and the production of "soluble specific substance" are also(More)
As determined by the intracutaneous test in guinea pigs, diphtheria toxin is not altered in the presence of cardiac tissue obtained from the fetal or from the adult heart of the guinea pig. Tissue cultures were apparently uninjured by the presence of the toxin in the dilutions used in these experiments, and, when washed with embryo extract after removal of(More)
The potency of a polyvalent antimeningococcus serum, as tested by its agglutination titer, was sacrificed by immunization with a large number of strains of the meningococcus. By immunization with a limited number of representative strains, four or six, carefully selected on account of their antigenic and agglutination properties, the potency was increased(More)
The meningococcus, like some other pathogenic species, varies in its agglutination in immune serum, some strains being readily agglutinable while others agglutinate with difficulty in their homologous serum as well as in heterologous serums. The different strains appear to vary also in their action as antigens. In order to secure representative strains,(More)
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