Atsushi Kubo

Learn More
Introducing a point mutation is a fundamental method used to demonstrate the roles of particular nucleotides or amino acids in the genetic elements or proteins, and is widely used in in vitro experiments based on cultured cells and exogenously provided DNA. However, the in vivo application of this approach by modifying genomic loci is uncommon, partly due(More)
  • Atsushi Kubo, Robert Stull, Mitsuaki Takeuchi, Kristina Bonham, Valerie Gouon-Evans, Masayuki Sho +4 others
  • 2011
In order to define the molecular mechanisms regulating the specification and differentiation of pancreatic β-islet cells, we investigated the effect of upregulating Pdx1 and Ngn3 during the differentiation of the β-islet-like cells from murine embryonic stem (ES) cell-derived activin induced-endoderm. Induced overexpression of Pdx1 resulted in a significant(More)
At near ambient conditions, CaIrO 3 is isostructural with the high-pressure polymorph MgSiO 3 " post-perovskite " (pPv). MgSiO 3 pPv is thought to be a major phase in the earth's lowermost mantle. CaIrO 3 can thus serve as an analog for studying deformation of the pPv phase under conditions achievable with a multi-anvil deformation apparatus. Here we study(More)
Ascidians belong to the subphylum Urochordata or Tunicata, which is the sister group of the vertebrates. The simple architecture of the ascidian larva represents the basic chordate body plan. Recent analyses have shown many instances of developmental mechanisms conserved during evolution, while these studies have also revealed a much larger number of(More)
Estuarine and shallow coastal systems (ESCS) are recognized as not only significant organic carbon reservoirs but also emitters of CO2 to the atmosphere through air-sea CO2 gas exchange, thus posing a dilemma on ESCS's role in climate change mitigation measures. However, some studies have shown that coastal waters take up atmospheric CO2 (Catm), although(More)
Maternal factors initiate the zygotic developmental program in animal embryos. In embryos of the chordate, Ciona intestinalis, three maternal factors-Gata.a, β-catenin, and Zic-r.a-are required to establish three domains of gene expression at the 16-cell stage; the animal hemisphere, vegetal hemisphere, and posterior vegetal domains. Here, we show how the(More)
AIMS/INTRODUCTION Patients with diabetic nephropathy (DN) typically show varying degrees of proteinuria and renal impairment. Because these clinical signs are frequently observed in other glomerulopathies, renal biopsy is required to make a definitive diagnosis of DN. We carried out the present study to evaluate the significance of renal biopsy for patients(More)
We have recently constructed a web-based database of gene expression in the mouse whole embryo, EMBRYS ( To allow examination of gene expression patterns to the fullest extent possible, this database provides both photo images and annotation data. However, since embryos develop via an intricate process of(More)
Development of factor VIII (fVIII)-neutralizing antibodies, called inhibitors, is a challenging problem in the management of hemophilia A patients. We explored the possibility of pretreatment with apoptotic fVIII-expressing embryonic stem (ES) cells to prevent the development of fVIII inhibitors. Murine ES cells integrated with the human F8 gene were(More)
PURPOSE It is useful to convert free-text diagnostic reports into structured diagnostic reports by semantic analysis for the secondary investigation of their contents. In this study, we propose a system in which description units are automatically extracted to create structured text reports and we evaluated its usefulness. METHODS We defined the rules to(More)