Atsushi Koike

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In fission yeast, calcineurin dephosphorylates and activates the Prz1 transcription factor. Here, we identified the calcineurin-dependent response element (CDRE) in the promoter region of prz1 gene, and monitored the calcineurin activity in living cells using a destabilized luciferase reporter gene fused to three tandem repeats of CDRE. Elevated(More)
In fission yeast, calcineurin dephosphorylates and activates the Prz1 transcription factor. Here, we identified the calcineurin-dependent response element (CDRE) in the promoter region of prz1(+) gene and monitored the calcineurin activity in living cells using a destabilized luciferase reporter gene fused to three tandem repeats of CDRE. Elevated(More)
Fission yeast Schizosaccharomyces pombe is amenable to genetics and is an excellent model system for studying eukaryotic cell biology. However, auxotrophic markers that can be used for both targeted gene integration and disruption are very limited. Here we performed a forward genetic screen in an effort to develop a new set of selectable markers for use in(More)
Motion JPEG2000 has been developed as a new video coding standard for motion pictures, utilizing the still image coding standard JPEG2000. Motion JPEG2000 specifies a normative bitstream syntax that the decoder must recognize and allows flexible selection of detailed coding parameters at the encoder. The compression performance largely depends, therefore,(More)
We propose a novel computational model for GPU. Known parallel computational models such as the PRAM model are not appropriate for evaluating GPU algorithms. Our model, called AGPU, abstracts the essence of current GPU architectures such as global and shared memory, memory coalescing and bank conflicts. We can therefore evaluate asymptotic behavior of GPU(More)
This paper proposes a novel CT image coding concept named LOI which stands for level of interest and coding methods based on that concept. The well-known ROI (region of interest) coding gives priority to pixels in particular regions of an image when encoding or decoding the image. Similarly, LOI coding gives priority to the pixels having a particular pixel(More)
We propose a novel computational model for GPUs. Known parallel computational models such as the PRAM model are not appropriate for evaluating GPU-based algorithms. Our model, called AGPU, abstracts the essence of current GPU architectures such as global and shared memory, memory coalescing and bank conflicts. Using our model, we can evaluate asymptotic(More)
The regulation of cytoplasmic Ca(2+) is crucial for various cellular processes. Here, we examined the cytoplasmic Ca(2+) levels in living fission yeast cells by a highly sensitive bioluminescence resonance energy transfer-based assay using GFP-aequorin fusion protein linked by 19 amino acid. We monitored the cytoplasmic Ca(2+) level and its change caused by(More)