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Withania somnifera, also known as Indian ginseng is known to contain valuable bioactive compounds, called withanolides that structurally resemble ginsenosides of Panax ginseng. These compounds provide the basis of pharmacological relevance in traditional systems of medicine. In the present study, 150 hairy root lines of W. somnifera were induced of which(More)
Artemisinin (AN) and artemisinic acid (AA), valuable phyto-pharmaceutical molecules, are well known anti-malarials, but their activities against diseases like cancer, schistosomiasis, HIV, hepatitis-B and leishmaniasis are also being reported. For the simultaneous estimation of AN and AA in the callus and leaf extracts of A. annua L. plants, we embarked(More)
Withania somnifera, is an important medicinal plant in India, also known as Ashwagandha, that contains the bioactive compounds- withanolides and withaferin A. The endophytic fungus Piriformospora indica has been shown to be an elicitor stimulating plant growth and metabolism. Different concentrations of cell homogenate, culture filtrate and individual(More)
The possibility of using PCR to specify the detection of aflatoxigenic fungi in food and feeds was investigated. The method, based on amplification of the aflP gene encoding the biosynthesis of aflatoxins, was optimized for the detection of aflatoxin-producing molds (Aspergillus flavus MTCC 277, A. flavus JH 11 and A. parasiticus MTCC 2796). The specificity(More)
INTRODUCTION Herbals drugs became a boon for mankind since ancient times and still are used worldwide for the treatment of various human ailments. The safety of alternative medicinal preparations has been questioned due to reports of unwanted side effects. To maintain the quality and efficacy of these drugs, it is essential to standardize them in order to(More)
miRNAs are emerging as potential regulators of the gene expression. Their proven promising role in regulating biosynthetic pathways related gene networks may hold the key to understand the genetic regulation of these pathways which may assist in selection and manipulation to get high performing plant genotypes with better secondary metabolites yields and(More)
The date seeds oil is a mixture of different types of major and minor organic components with potential positive health benefits. Thus, the study was designed to conversion of Ajwa verity of Saudi Arabian date seeds into pyrolysis liquid char oil by slow pyrolysis reactor and its phytoconstituents were determined by GC–MS. The date seed in particle form was(More)
A broad screening protocol, covering the most general phytochemical groups of compounds, was developed on the basis of high performance thin layer chromatography (HPTLC). A total of six TLC systems, comprising three derivatization reagents, two stationary phases and two mobile phases, were included. The screening method was applied for the identification of(More)
Ashwagandha (Withania somnifera) is one of the most reputed medicinal plants in the traditional medicinal system. In this study, cell suspension culture of W. somnifera was elicited with cell homogenates of fungi (A. alternata, F. solani, V. dahliae and P. indica) in shake flask and the major withanolides like withanolide A, withaferin A and withanone were(More)
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