Asuka Nakano

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Protein dielectrophoresis (DEP) has the potential to play an important role as a manipulation, fractionation, preconcentration, and separation method in bioanalysis and as manipulation tool for nanotechnological applications. The first demonstrations of protein DEP have been reported almost 20 years ago. Since then various experimental realizations to(More)
The effect of clipping of the host-plant shoot on the sources of carbon and nitrogen for the arbuscular mycorrhizal (AM) fungus Gigaspora margarita was determined by measuring 13C in spores and hyphae in cocultures of C3 and C4 plants and by differential 15N labeling. C3 and C4 plants, which have different δ13C values, were grown in the same container(More)
Dielectrophoresis (DEP) of biomolecules has large potential to serve as a novel selectivity parameter for bioanalytical methods such as (pre)concentration, fractionation, and separation. However, in contrast to well-characterized biological cells and (nano)particles, the mechanism of protein DEP is poorly understood, limiting bioanalytical applications for(More)
Fish gelatin (FG) extracted from sea bream scales was reacted with glycidyl methacrylate (GMA), and the product (FG-GMA) was used for photopolymerization using a radical photoinitiator in the presence or absence of imogolite nanofibers in the aqueous solution. The synthesis of FG-GMA was confirmed by H NMR spectroscopy, and photopolymerization of FG-GMA was(More)
Dielectrophoresis (DEP) has demonstrated to be a versatile tool to manipulate micro- and nanoparticles with applications for positioning, separation and fractionation. Recent developments of DEP have also shown that DEP can be used for the manipulation of biomolecules, such as DNA. Here, we focus on the manipulation of proteins using insulator-based(More)
The source of nitrogen in the spores of arbuscular mycorrhizal (AM) fungi was quantified by a 15N-labeling technique. N was applied as coated urea to the soil and in solution to plant shoots. Soil-applied fertilizer had a significant effect on spore % 15N (P<0.01), with a 24–75% contribution to spore N. Fertilizer applied to either alfalfa shoots or bahia(More)
Insulator-based dielectrophoresis (iDEP) has been explored as a powerful analytical technique in recent years. Unlike with larger entities such as cells, bacteria or organelles, the mechanism of iDEP transport of proteins remains little explored. In this work, we extended the pool of proteins investigated with iDEP in nanostructured devices with(More)
 The origin of carbon in the spores of arbuscular mycorrhizal (AM) fungi was quantified based on their obligate symbiosis with C3 and C4 plants showing clearly different δ13C values. The δ13C values of individual spores of the AM fungus Gigaspora margarita were analyzed. In monoculture pots of a C3 or a C4 plant species, spore δ13C values were ca. 3.5‰(More)
Insulator-based dielectrophoresis is a relatively new analytical technique with a large potential for a number of applications, such as sorting, separation, purification, fractionation, and preconcentration. The application of insulator-based dielectrophoresis (iDEP) for biological samples, however, requires the precise control of the microenvironment with(More)
Poster Title Page AES-1 Characterizing the Dielectric Properties of Human Mesenchymal Stem Cells Using a Quadrupole Microfluidic Device Tayloria Adams, Brenda Ogle, and Adrienne Minerick 2 AES-2 Monitoring Hydroxyl Radical Production In Mitochondria with Micellar Electrokinetic Chromatography Margaret A. Donoghue and Edgar A. Arriaga 3 AES-3 Separation of(More)
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