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Polycomb group (PcG) proteins are essential regulators of hematopoietic stem cells. Recent extensive mutation analyses of the myeloid malignancies have revealed that inactivating somatic mutations in PcG genes such as EZH2 and ASXL1 occur frequently in patients with myelodysplastic disorders including myelodysplastic syndromes (MDSs) and(More)
5-Hydroxymethylcytosine ((hm)C) is an essential intermediate in the active DNA demethylation pathway. Here we report a new base-resolution method for measuring (hm)C by combining peroxotungstate-mediated oxidation and sequencing analysis. We reveal that an oxidized product of (hm)C, trihydroxylated thymine ((th)T), tolerated the incorporation of dATP as a(More)
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