Ashok K Mishra

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The FecB gene of Garole sheep was introgressed into non-prolific Malpura sheep to evolve a new prolific sheep strain Garole × Malpura (GM), suitable for semi-arid conditions. The present study was conducted to evaluate the impact of breeding program on production profile of GM sheep and to estimate the genetic parameters for growth traits of GM sheep.(More)
The extent to which farm families engage in off-farm employment activities is a topic of central importance to understanding the financial well-being of farm households. The degree of financial stress experienced by U.S. farm families is always a concern to policy makers. Yet, policy considerations often ignore the substantial involvement of most farm(More)
This analysis utilizes farm-level data to evaluate the extent to which U.S. farm program benefits, particularly direct payments, bring about distortions in production. The issue is important in WTO negotiations and in the debate over the distortionary effects of decoupled (“green-box”) payments. Our results suggest that the distortions brought about by AMTA(More)
An indirect fluorescent antibody test (IFAT) and slide enzyme linked immunosorbent assay (SELISA) were standardized for the detection of antibodies specific to Babesia bigemina in experimentally infected bovine calves and subsequently used for the screening of naturally infected bovine and bubaline sera. In experimentally infected calves positive reactivity(More)
BACKGROUND An epidemiological and entomological study was carried out in Balaghat district, Madhya Pradesh, India to understand the dynamics of forest malaria transmission in a difficult and hard to reach area where indoor residual spray and insecticide treated nets were used for vector control. METHODS This community based cross-sectional study was(More)
An antibody detection recombinant enzyme-linked immunosorbent assay (ELISA) specific for Toxoplasma gondii was laboratory standardized using recombinant truncated surface antigen 2 (SAG2) protein of T. gondii. A 483-bp sequence coding for truncated tachyzoite stage-specific SAG2 protein was amplified and ligated in pPROExHT-b expression vector to transform(More)
Cloned populations were generated from Indian isolates of Toxoplasma gondii by transferring single tissue cysts from the brains of chronically infected mice to confluent murine macrophage (J774A.1) monolayers. The clones were then maintained continuously as tachyzoites in culture. Physical rupture of the tissue cysts and release of bradyzoites prior to(More)
Additives like salts and surfactants can alter the phase transition temperature of poly(N-isopropylacrylamide) (PNIPAM). The inclusion of a biological surfactant like sodium cholate (NaC) into PNIPAM could lead a better biocompatibility when the materials are used for biomedical applications. The phase transition behavior of PNIPAM was studied in presence(More)