Arun M. Khurad

Learn More
Nucleopolyhedrovirus (NPV) was tested for vertical transmission in the silkworm, Bombyx mori. Fifth instar larvae were exposed to four different dosages of BmNPV (830, 1300, 1800, and 2000OBs/larva) and a dosage of about 2000OBs/larva was found suitable for obtaining infected adults. Histopathological studies revealed the infection in susceptible tissues(More)
A new continuous cell line from ovarian tissue of commercial variety “Kolar Gold” of silkworm, Bombyx mori, was established and designated as DZNU-Bm-12. The tissue was grown in MGM-448 insect cell culture medium supplemented with 10% fetal bovine serum (FBS) and 3% heat-inactivated B. mori hemolymph at 25 ± 1°C. The migration of partially attached small(More)
Lepidopteran cell lines constitute the backbone for studying baculoviral biology in culturo and for baculovirus vector based recombinant protein expression systems. In the present study, we report establishment of a new continuous cell line designated as DZNU-Bm-1 from larval ovaries of the silkworm, Bombyx mori. The cells were grown in MGM-448 insect cell(More)
Nosema locustae, a microsporidian parasite of locusts and grasshoppers, was successfully propagated in a fat body cell line from Mythimna convecta (BPMNU-MyCo-1). The fat body cells were grown in MGM-448 medium supplemented with 5% fetal bovine serum and 3% Bombyx mori serum at 25 degrees C. Cultures were inoculated with Nosema spores and agitated for 2(More)
Nosema locustae, a microsporidian parasite of locusts and grasshoppers, was transovarially transmitted to the progeny of infectedLocusta migratoria reared for up to F14 generations. The mortality of infected progeny in each generation was higher than that of uninfected controls and ranged from 67.6% to 95.5%. Infected female survivors transmitted the(More)
The neurosecretory cells of the pars intercerebralis and ring gland are examined in histological sections of larva, pupa and adult of buffalo-fly, Lyperosia exigua fixed at various intervals during the rpost-embryonic development. Three paired medial groups of the neurosecretory cells are observed in the larval brain. These groups show their displacement(More)
In the adult buffalo-fly, L. exigua three groups of neurosecretory cells are present in each half of the brain; medial and lateral groups in the protocerebrum and ventral group in the tritocerebral region. The cerebral neurosecretory cells are classified as A and B cells. Both the cells are present in the medial, lateral and ventral groups. Histochemically,(More)
This study examines butterfly larval host plants, herbivory and related life history attributes within Nagpur City, India. The larval host plants of 120 butterfly species are identified and their host specificity, life form, biotope, abundance and perennation recorded; of the 126 larval host plants, most are trees (49), with fewer herbs (43), shrubs (22),(More)
The neurosecretory cells of pars intercerebralis, the corpus cardiacum and the corpus allatum are examined in histological sections of Lyperosia exigua females, fixed at daily interval during first cycle of egg maturation. The median neurosecretory A cells exhibit secretory activity by undergoing cyclical changes of synthesis and release with the advancing(More)
  • 1