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The quantitation of thyroid hormone levels in cerebrocortical synaptosomes of the adult rat at altered thyroid states was studied because of the recent idea of the involvement of thyroid hormone in the adult mammalian brain. In contrast to a marked fall in serum T3 levels in PTU-induced hypothyroid rats, the synaptosomal T3 content was raised by 9.5 fold in(More)
Interactions of L-triiodothyronine (T3) in adult rat cerebrocortical synaptosomes were studied in vitro. Scatchard plot analysis revealed two sets of T3 binding sites. The degree of saturation of T3 binding sites (putative receptor) correlated well with the dose-dependent inhibition of Na(+)-K(+)-ATPase activity in synaptosomes. The relative binding(More)
BACKGROUND Imbalance in thyroid hormone concentrations has been linked with profound neurobehavioral alterations in the adult. Peripheral hypothyroidism is associated with a phenomenon of central thyroid hormone homeostasis in adult rat. This central homeostasis mechanism could be maintained by adrenergic interplay due to close physiological association(More)
Thyroid hormones are essential for normal functioning of adult mammalian brain. The present investigation deals with the understanding of the time course of thyroid hormone homeostasis in adult rat brain. Animals were rendered hypothyroid by PTU injections (2 mg/100 g bw) for 30 consecutive days. Serum and synaptosomal T3/T4 content, synaptosomal AChE and(More)
Accumulation of free, ionized calcium (Ca2+) and stimulation of nitric oxide synthase (NOS) activity in depolarization-induced synaptosomes prepared from adult rat cerebral cortex have been demonstrated after addition of various doses (0.1-1,000 nM) of 3,5,3'-L-triiodothyronine (T3). The effects of T3 doses on those parameters are found to occur in a(More)
Isolation and enumeration of phytase-producing bacteria in the proximal intestine (PI) and distal intestine (DI) of four freshwater teleosts, Nile tilapia (Oreochromis niloticus), murrel (Channa punctatus), climbing perch (Anabas testudineus), and stinging catfish (Heteropneustes fossilis) have been carried out following enrichment culture technique. The(More)
In view of the lack of knowledge on the thyroid hormone (T3) action in adult mammalian brain the present investigation was undertaken to assess the role of this hormone on synaptosomal function of adult rat cerebral cortex. Single injection of various doses of T3 showed dose-dependent inhibition of the synaptosomal Na(+)-K(+)-ATPase activity with its(More)
A simple and rapid low speed density gradient centrifugation method has been described to isolate relatively pure synaptosomes and non-synaptic mitochondria from rat brain cerebral cortex. The purity of the fractions isolated were tested biochemically by considering some marker enzymes, i.e., lactate dehydrogenase, plasma membrane Na+K+ATPase and(More)
Despite the recently emerging notion of thyroid-hormone involvement in neurotransmission in the adult mammalian brain, adequate evidence for a cellular basis of the process is still lacking. The present study indicates the involvement of thyroid hormones in cholinergic system of the adult rat cerebral cortex. Administration of L-triiodothyronine (T3, 0.025(More)