Arnošt Kotyk

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Recommendations on glycolipid nomenclature have been approved, and are published in Carbohydr. Res. 312, 167-175 0998); Eur.J.Biochem. 257, 293-298 (I998); Glycoconjuqate J. 16 0999); J.MoLBiol. 286, 963-970 (~999); Pure AppL Chem. 69, 2475-2487 (x997), and are available at ( w w w . c h e m . q m w . a c . u k / i u p a c / m i s c / g l y l p . h t m l )(More)
A late exponential culture, cultivated in the absence of phosphates, and a similar culture supplied with phosphate (phosphate overcompensation conditions) were prepared from an industrial strain of Saccharomyces cerevisiae. For the cytochemical staining, the cellular phosphates were transformed into polymeric metal-phosphate complexes by Ca2+ and Mg2+ ions,(More)
The lager brewing yeasts, Saccharomyces pastorianus (synonym Saccharomyces carlsbergensis), are allopolyploid, containing parts of two divergent genomes. Saccharomyces cerevisiae contributed to the formation of these hybrids, although the identity of the other species is still unclear. The presence of alleles specific to S. cerevisiae and S. pastorianus was(More)
Ornithine carbamoyltransferase (OCT) from spinach (Spinacea oleracea L.) was purified to homogeneity and studied for some kinetic and structural properties. The enzyme showed a specific activity of 436 U mg−1, its molecular mass was approximately 118 kDa as estimated by Sephacryl S-200 gel filtration chromatography, the purified protein ran as a single band(More)
The steady-state levels of distribution of glycine,l-aspartic acid,l-leucine and, to a lesser extent, ofl-lysine andl-methionine, in actidione-treated baker’s yeast cells are significantly altered (usually decreased) in the presence ofd-glucose,d-mannose,d-fructose, 2-deoxy-d-glucose, maltose, sucrose and, after induction,d-galactose. Stimulatory effects(More)
Experiments with respiration deficient (rho-), ADP/ATP transport deficient (op1) and double (op1 rho-) mutants, with glycolytic and tricarboxylic acid cycle substrates showed that the substrate-induced acidification of yeast suspensions is closely associated with glycolysis. The glucose/proton stoichiometry is 2.5 : 1 to 4 : 1 depending on glucose(More)
The membrane potential, delta psi, of various yeasts estimated from the distribution of tetraphenylphosphonium cations ranged from -50 to -120 mV, depending on species, incubation conditions and technique of measurement. Values obtained directly with a microelectrode in Endomyces magnusii were consistently lower than those determined indirectly.