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  • A D Bastos
  • 1998
Genomic amplification of the VP1 gene of SAT-type foot-and-mouth disease virus (FMDV) was performed with published and novel oligonucleotide primers. The primer pair with the highest SAT-type recognition (67%) was identified and selected for optimization. Modifications to primers significantly improved SAT-type detection (100%), broadened the recognition(More)
VP1 gene nucleotide sequences of 51 SAT3-type foot-and-mouth disease (FMD) viruses from seven southern and eastern African countries were used to infer a gene phylogeny. Results obtained by phylogenetic analysis of the homologous 405 nt region corresponding to the C-terminal 128 amino acids of 1D and adjacent 7 amino acids of 2A indicate that there are six(More)
The nature of selection on capsid genes of foot-and-mouth disease virus (FMDV) was characterized by examining the ratio of nonsynonymous to synonymous substitutions in 11 data sets of sequences obtained from six different serotypes of FMDV. Using a method of analysis that assigns each codon position to one of a number of estimated values of nonsynonymous to(More)
Genetic relationships of serotype O foot-and-mouth disease (FMD) viruses recovered from outbreaks of the disease in the West African countries of Niger, Burkina Faso and, Ghana (1988–1993) and those from South Africa (2000) were determined by partial VP1 gene characterization. A 581-bp fragment, corresponding to the C-terminus half of the 1D (VP1 gene)(More)
protein [11]). These latter values are due to normal, anaerobic glycolysis in the lens. The high values in Phelsuma indicate that e-crystallin is expressed as an active enzyme, similar to the situation in birds. It has been proposed that the recruitment as crystallins of NAD(P)H-binding enzymes, which causes increased levels of reduced pyridine nucleotides,(More)
African swine fever (ASF) viruses are characterised by numerous p72 genotypes, but by low levels of intra-genotypic variation, particularly in domestic pig associated genotypes. As it is precisely these viral lineages that are involved in outbreaks of the disease it is imperative that alternative, more informative gene regions be identified which are(More)
A character-selection procedure initially applied in vertebrates (viverrid carnivores and murid rodents), but with a potential, more general application, was used to select appropriate characters for a morphometric investigation of weevils on Marion Island. An initial set of 23 linear measurements, adopted from a previous morphometric study, was subjected(More)
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