Aris Tri Wahyudi

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Magnetospirillum sp. strain AMB-1 is a Gram-negative alpha-proteobacterium that synthesizes nano-sized magnetites, referred to as magnetosomes, aligned intracellularly in a chain. The potential of this nano-sized material is growing and will be applicable to broad research areas. It has been expected that genome analysis would elucidate the mechanism of(More)
Problem statement: Pseudomonas spesies are one of the rihizobacterial group that have an important role in plant growth promoter and plant health. To prepare them as inoculants, they must have a range of characters as growth promoter such as Indole Acetic Acid (IAA) producers which can promote the growth of plants and solubilize phosphates. In addition,(More)
Sponges associated with microorganism can produce bioactive compounds such as antioxidant and anticancer. The aim of this study determines the toxicity, anticancer and antioxidant potency of marine bacteria which associated with sponges Jaspis sp. The bioactive compounds were extracted with ethyl acetate. The toxicity of extract was analyzed trough by brine(More)
A 3.7-kb cryptic plasmid designated pMGT was found in Magnetospirillum magneticum MGT-1. It was characterized and used for the development of an improved expression system in strain AMB-1 through the construction of a shuttle vector, pUMG. An electroporation method for magnetic bacteria that uses the cryptic plasmid was also developed.
Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 x 10(-7) per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events(More)
A non-magnetic mutant, NMA61, of the magnetic bacterium Magnetospirillum magneticum AMB-1 was generated by transposon mutagenesis to identify genes involved in magnetosome synthesis. The genomic region of NMA61 interrupted by a Mini-Tn5 transposon was analyzed. The transposon was inserted in an open reading frame (ORF) coding for a periplasmic transport(More)
Fungal leaf blast, caused by Pyricularia oryzae, is a devastating disease of rice plants that annually causes severe production losses worldwide and is one of the top 10 fungal diseases that threaten global food security. Thus, a reliable control strategy against this disease is essential. In this study, the antagonistic activity of indigenous phyllosphere(More)
Insulin-like growth factor-1 (Igf1) plays important roles in fish growth, development and metabolism. The aims of this study were to isolate, characterize, and obtain a set of real-time PCR primers of giant gourami (Osphronemus goramy) Igf1-like gene. We used reverse transcription and rapid amplification of cDNA ends (RACE) to obtain the giant gourami(More)
A non-magnetic mutant of Magnetospirillum magneticum AMB-1, designated as NMA21, was generated by mini-Tn5 transposon mutagenesis to identify genes involved in bacterial magnetic particle (BMP) synthesis. Alignment of the DNA sequences flanking the transposon allowed the isolation of an open reading frame (ORF2) within an operon consisting of five genes.(More)
Corresponding Author: Aris Tri Wahyudi Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Indonesia Phone/Fax: +62-251-8622833 Email: aristri2011@gmail.com Abstract: Sponge-associated bacteria have great potential in developing marine pharmaceutical industry since they are capable of synthesizing numerous(More)
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