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Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered theExpand
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Organization and dynamics of the Aspergillus nidulans Golgi during apical extension and mitosis.
Aspergillus nidulans hyphae grow exclusively by apical extension. Golgi equivalents (GEs) labeled with mRFP-tagged PH(OSBP) domain form a markedly polarized, dynamic network of ring-shaped andExpand
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Long‐Distance Movement of Aspergillus nidulans Early Endosomes on Microtubule Tracks
In fungal hyphal cells, intracellular membrane trafficking is constrained by the relatively long intracellular distances and the mode of growth, exclusively by apical extension. Endocytosis plays aExpand
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Maturation of late Golgi cisternae into RabERAB11 exocytic post-Golgi carriers visualized in vivo
Maturation of Golgi cisternae into post-Golgi carriers is directly visualized using the Aspergillus nidulans Ypt31/RAB11 homologue RabE as a reporter of post-Golgi identity. A microtubule-basedExpand
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The nucleobase-ascorbate transporter (NAT) signature motif in UapA defines the function of the purine translocation pathway.
UapA, a member of the NAT/NCS2 family, is a high affinity, high capacity, uric acid-xanthine/H+ symporter of Aspergillus nidulans. We have previously presented evidence showing that a highlyExpand
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Endosomal maturation by Rab conversion in Aspergillus nidulans is coupled to dynein-mediated basipetal movement
Highly motile fungal early endosomes can be easily distinguished from more static late endosomes and vacuoles, a feature that is exploited to study endosomal maturation. RabA/RabB early endosomesExpand
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Mutational analysis and modeling reveal functionally critical residues in transmembrane segments 1 and 3 of the UapA transporter.
Earlier, we identified mutations in the first transmembrane segment (TMS1) of UapA, a uric acid-xanthine transporter in Aspergillus nidulans, that affect its turnover and subcellular localization.Expand
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Specific interdomain synergy in the UapA transporter determines its unique specificity for uric acid among NAT carriers.
UapA, a uric acid-xanthine permease of Aspergillus nidulans, has been used as a prototype to study structure-function relationships in the ubiquitous nucleobase-ascorbate transporter (NAT) family.Expand
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Aspergillus RabBRab5 Integrates Acquisition of Degradative Identity with the Long Distance Movement of Early Endosomes
Of the two Aspergillus early endosomal Rab5 paralogues, RabB recruits, in its GTP conformation, Vps19, Vps45, and Vps34, and the CORVET complex and couples acquisition of PI(3)P degradative identityExpand
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Acute inactivation of the Aspergillus nidulans Golgi membrane fusion machinery: correlation of apical extension arrest and tip swelling with cisternal disorganization
The mechanisms governing traffic across the Golgi are incompletely understood. We studied, by live‐cell microscopy, the consequences of disorganizing the Aspergillus nidulans Golgi, using an extendedExpand
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