Ara P. Antonyan

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The pH-induced helix-coil transition of DNA and its complexes with EtBr is carried out at acidic pH in a wide interval of change of concentration ratio of EtBr/DNA. The binding isotherms of EtBr on double and single-stranded DNA at pH = 7.0 and pH = 3.0 (t = 25 °C) are obtained by absorption and fluorimetric methods. Binding constants (K) and number of(More)
The helix-coil transition of DNA-ethidium bromide complexes in an interval of ionic strength of 2.0 x 10(-3) M ≤ muNa+ ≤ 2.0 x 10(-2) M has been investigated. It has been revealed that at the certain high ligand-DNA ratios (r(b)) the transition interval of the complex-(ΔT) becomes equal to that of DNA itself (ΔTo). It has been shown that the values of r(b)(More)
The binding of Hoechst 33258 with DNA at various ionic strengths of solution and different ligand concentrations has been investigated. Existence of more than one type of interactions of Hoechst 33258 with DNA has been revealed, which were very sensitive to the ionic strength. Hoechst 33258 doesn't show specificity to AT sequences of DNA at low ionic(More)
The thermostability and density of water-salt solutions of DNA, irradiated by non thermal coherent millimeter electromagnetic waves with frequency 64.5 GHz have been investigated using the methods of spectrophotometry and densitometry. It is shown that the thermostability of DNA and density of its solutions are increased, depending on time of irradiation.(More)
Spectrophotometric study of Hoechst 33258 binding to DNA has been investigated. It was shown that this ligand forms at least two types of complexes at 0.002 M and 0.02M ionic strengths of solution. One of them is strong, the other is weak. It was revealed that at 0.02M ionic strength of solution, the binding constant value by strong mode is one order higher(More)
Interaction of intercalators: ethidium bromide (EtBr), methylene blue (MB) and acridine orange (AO), with DNA at 0.02 M ionic strength of the solution has been studied by differential spectroscopy method. It was revealed that EtBr binds to DNA by intercalation, semi-intercalation and electrostatic modes; MB by semiintercalation and electrostatic modes and(More)
146 The binding mechanisms of methylene blue with DNA Ara P. Antonyan, Lilit A. Hambardzumyan, Marine A. Parsadanyan & Poghos O. Vardevanyan To cite this article: Ara P. Antonyan, Lilit A. Hambardzumyan, Marine A. Parsadanyan & Poghos O. Vardevanyan (2015) 146 The binding mechanisms of methylene blue with DNA, Journal of Biomolecular Structure and Dynamics,(More)
Os parâmetros de ligação de brometo de etídio (EtBr) com DNA em diversas proporções GC/AT foram determinados usando espectroscopia de absorção e fluorescência. Nossos dados experimentais demonstram claramente a co-existência de tipos de ligação “forte” fluorescente e não-fluorescente em baixas concentrações de EtBr. O complexo fluorescente corresponde ao(More)