Learn More
An efficient protocol for direct shoot organogenesis has been developed for the medicinal plant Aerva lanata (L.) Juss. ex Schult. Regeneration was achieved from leaf segments of 20 days old in vitro plantlets raised on Murashige and Skoog (MS) medium containing 0.25-2.0 mg L(-1) thiadiazuron (TDZ), 3% sucrose, and 0.8% agar. After 21 days of culture(More)
A reproducible protocol for somatic embryogenesis was established for mango ginger (Curcuma amada Roxb.)—an important horticultural aromatic rhizomatous plant. Embryogenic callus induction was obtained from leaf sheath explants of in vitro raised plants on Murashige and Skoog (MS) agar medium containing 2.0 mg/L 2,4-dichlorophenoxyacetic acid and 0.5 mg/L(More)
An efficient and simple procedure was systematically developed for inducing direct somatic embryogenesis and plantlet regeneration from leaf sheath explants of Curcuma amada Roxb. A two-step culture system was used to induce somatic embryogenesis. The optimized procedure resulted in direct somatic embryogenesis from 93.3% explants after 3-wk culture. Leaf(More)
This study describes for the first time a simple and quick direct somatic embryogenesis and plant regeneration protocol for turmeric (Curcuma longa L.) using leaf base explants. Preincubation of explants was carried out on solid Murashige and Skoog (MS) medium containing 4.49 μM 2,4-dichlorophenoxy acetic acid for 20 days under continuous dark condition.(More)
We developed a protocol to produce synthetic seeds of mango ginger (Curcuma amada Roxb.) for large-scale propagation, storage, and germplasm exchange. The seeds were produced by encapsulating somatic embryos in a calcium–alginate matrix. Embryogenic callus was induced from leaf sheath explants on 0.8% agar solidified with full-strength Murashige and Skoog(More)
The " International Journal of Pharma and Bio Sciences " (IJPBS) is an international journal in English published quarterly. The aim of IJPBS is to publish peer reviewed research and review articles rapidly without delay in the developing field of pharmaceutical and biological sciences And indexed/catalogued in many more university
An improved Agrobacterium rhizogenes-mediated genetic transformation of Withania somnifera (L.) Dunal was developed using the bacterial strain R1000 with leaf segment explants of in vitro raised plantlets. Out of the three strains used (R1000, MTCC 2364 and MTCC 532), the strain R1000 proved to be more efficient than others. Among the different conditions(More)
  • 1