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An antibody toolkit for the study of membrane traffic in Drosophila melanogaster

It is shown that the monoclonal antibodies are highly specific and sufficiently sensitive to detect endogenous proteins in crude extracts by immunoblotting with little background staining and will provide a useful tool kit to facilitate the investigation of how the endomembrane system functions and varies in the diverse tissue types of metazoans.
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The two TRAPP complexes of metazoans have distinct roles and act on different Rab GTPases

It is shown that Drosophila melanogaster have two TRAPP complexes similar to those in mammals and that both activate Rab1, whereas one, TRAPPII, also activates Rab11 and is shared byTRAPPII and an unrelated GEF that is metazoan specific.
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Long‐Distance Movement of Aspergillus nidulans Early Endosomes on Microtubule Tracks

It is shown that the SynA synaptobrevin endocytic recycling cargo traffics through this region, which strongly supports the contention that polarized hyphal growth involves the association of endocyt recycling with the plus ends of MTs located at the tip, near the endocytics internalization collar.
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PalC, One of Two Bro1 Domain Proteins in the Fungal pH Signalling Pathway, Localizes to Cortical Structures and Binds Vps32

A likely S. cerevisiae orthologue of PalC has been identified, providing the basis for a unifying hypothesis of gene regulation by ambient pH in ascomycetes.
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Physiological Involvement in pH Signaling of Vps24-mediated Recruitment of Aspergillus PalB Cysteine Protease to ESCRT-III*S⃞

This work unambiguously positions the calpain-like protease PalB within the ESCRT-III-associated pH signaling complex, underlines the positive role of ESC RT-III in ambient pH signal transduction, and suggests a possible mechanism for PalB activation.
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Endosomal maturation by Rab conversion in Aspergillus nidulans is coupled to dynein-mediated basipetal movement

Highly motile fungal early endosomes can be easily distinguished from more static late endosomes and vacuoles, a feature that is exploited to study endosomal maturation. RabA/RabB early endosomes…
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Receptor-independent Ambient pH Signaling by Ubiquitin Attachment to Fungal Arrestin-like PalF*

It is shown here that PalF assists the plasma membrane localization of PalH and thatPalF overexpression slightly hypersensitizes the pathway to alkaline pH but does not bypass the need for the ambient pH signal receptor in signaling, and that expression of PalF-Ub leads to a considerable degree of signaling even in the complete absence of the receptor.
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An ordered pathway for the assembly of fungal ESCRT-containing ambient pH signalling complexes at the plasma membrane

It is demonstrated that Vps23–GFP, expressed at physiological levels, is also recruited to cortical structures, very conspicuous in vps27Δ cells, in a PalF- and alkaline pH-dependent manner, which strongly support a model in which pH signalling takes place in ESCRT-containing, plasma-membrane-associated, rather than endosome- associated, complexes.
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Aspergillus RabBRab5 Integrates Acquisition of Degradative Identity with the Long Distance Movement of Early Endosomes

Of the two Aspergillus early endosomal Rab5 paralogues, RabB recruits, in its GTP conformation, Vps19, Vps45, and Vps34, and the CORVET complex and couples acquisition of PI(3)P degradative identity…
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Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling

The data support a model in which certain ESCRT proteins are recruited to the plasma membrane to mediate pH signalling, and establish that Vps23, VPS36, Vps20 and Vps32 are essential for pH signalling.
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