Antonio Correia

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Five rRNA operons (rrn) were found by hybridization in the genome of 'Brevibacterium lactofermentum' ATCC 13869 and Corynebacterium glutamicum ATCC 13032. 'B. lactofermentum' DSM 20412 differed from the other corynebacteria tested in showing six hybridizing BamHI bands. Two of the rrn operons (rrnD and rrnE) were located in a single cosmid. Sequencing of(More)
A large number of species of corynebacteria are known to be amino acid producers, including members of the genera Corynebacterium and Brevibacterium. Pulsed-field gel electrophoresis (PFGE) of DNA fragments obtained by using endonucleases which recognize AT-rich hexanucleotide or octanucleotide sequences produces a discrete pattern of bands useful for(More)
A repetitive DNA element of the Gram+ Brevibacterium lactofermentum (Bl), cloned by a modification of the subtractive hybridization method, contained a 1.4-kb IS-like element, IS13869, which included an open reading frame (ORF) inside a perfect 26-bp terminal inverted repeat (TIR). An 8-bp direct repeat (DR) was found outside each TIR. The ORF encoded a(More)
Tributyltin (TBT) is a toxic compound employed in several industrial processes. Its discharge into the environment has been recognized worldwide as a pollution problem. The importance of bacteria in decontamination processes has been long acknowledged. The aim of this work was to screen TBT resistant/ degrading bacteria in order to investigate the involved(More)
A 6.0-kb SalI DNA fragment containing an entire rRNA operon (rrnB) was cloned from a cosmid gene bank of the phytopathogenic strain Rhodococcus fascians D188. The nucleotide sequence of the 6-kb fragment was determined and had the organization 16S rRNA-spacer-23S rRNA-spacer-5S rRNA without tRNA-encoding genes in the spacer regions. The 5' and 3' ends of(More)
BACKGROUND/AIMS Several treatment schedules have been tried in acute hepatitis C, but due to the heterogeneity of included patients and to the non-practical interferon protocols, we assessed prospectively in a non-randomized pilot study, the efficacy of interferon 2b. METHODOLOGY Five million units of interferon 2b were given three times weekly by(More)
The extension of a well-known inverse technique, inverse adding-doubling (IAD), is investigated for determining the volume scattering properties of diffusers for display and lighting applications. The luminance characteristics of volume scattering diffusers are vital for these applications. Through a simulation study, it is shown that fitting solely to the(More)
We present a methodology to assess cell level alterations on the human retina responsible for functional changes observable in the Optical Coherence Tomography data in healthy ageing and in disease conditions, in the absence of structural alterations. The methodology is based in a 3D multilayer Monte Carlo computational model of the human retina. The(More)