Antoni Iborra

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The aim of this study was to evaluate single and double centrifugation tube methods for concentrating equine platelets. Whole blood samples were collected from clinically normal horses and processed by use of single and double centrifugation tube methods to obtain four platelet concentrates (PCs): PC-A, PC-B, PC-C, and PC-D, which were analyzed using a flow(More)
Cholesterol efflux from membranes promotes acrosome reaction in goat spermatozoa. In 1 h of incubation of sperm in the presence of beta-cyclodextrin (beta CD), all the interchangeable cholesterol is desorbed from sperm membranes, although acrosome reaction is fully accomplished only after 3-4 h of incubation, as previously published. In the present paper we(More)
Polycystic ovary syndrome (PCOS) affects 5-10% of women of reproductive age. Free radicals, as a product of oxidative stress, impair cells and tissue properties related to human fertility. These free radicals, together with the oxidized molecules, may have a cytotoxic or deleterious effects on sperm and oocytes, on early embryo development or on the(More)
Cholesterol efflux and membrane destabilization play an important role in sperm capacitation and membrane fusion in the acrosome reaction (AR). In this study we establish the effect of cholesterol removal from spermatozoa on acrosomal responsiveness. Mature goat spermatozoa were incubated in BSA-free medium in the presence of beta-cyclodextrin (betaCD) as(More)
An appropriate local environment is necessary for successful implantation. Oxidative stress is implicated in the pathogenesis of several pathologies, and may contribute to early pregnancy failure. Antioxidant therapies have been studied in infertility. In this study, we have assessed the antioxidant activity of N-acetylcysteine (NAC), flavonoids (quercetin,(More)
There is convincing evidence that the establishment of a chronic inflammatory response, together with the presence of a local oxidative environment, could play an important role in the etiology and the progression of several human diseases. In the reproductive system, pathologies such as endometriosis, polycystic ovary syndrome, tubal obstruction,(More)
PROBLEM The purpose of this study was to investigate the frequency of anti-endometrial antibodies (AEA) in infertile women. METHOD OF STUDY Sera from fertile women (n = 6), and from patients with ovulatory dysfunction (n = 11), tubal obstruction (n = 9) and unexplained infertility (n = 5) were investigated for the presence of anti-endometrial membrane(More)
PROBLEM The aim of this study was to investigate the humoral immune response to the female reproductive tissues associated with endometriosis (grades I-III) (n = 52), compared with a group of healthy fertile women (n = 6). METHOD OF STUDY An ELISA with cultured endometrial cell lines in monolayer was used to determine the presence of anti-endometrial(More)
Spermadhesin AWN is a major protein of boar seminal plasma and a sperm surface-associated lectin. AWN binds to beta-galactosides and to porcine zona pellucida glycoproteins, suggesting a role for this protein in primary gamete interaction. However, because capacitation induces remodelling of the sperm surface and AWN is peripherally bound to the plasma(More)
Boar spermadhesin AWN-1 is a sperm surface-associated 14.7-kDa lectin and a major protein of porcine seminal plasma. AWN-1 binds to beta-galactosides and to porcine zona pellucida glycoproteins, suggesting that this protein might play a role in the primary binding of spermatozoa to the egg's external glycoprotein matrix. We have produced a collection of(More)