Anton S. Tremsin

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Two optical configurations are commonly used in single-molecule fluorescence microscopy: point-like excitation and detection to study freely diffusing molecules, and wide field illumination and detection to study surface immobilized or slowly diffusing molecules. Both approaches have common features, but also differ in significant aspects. In particular,(More)
The measurements of the electron cloud footprints produced by a stack of microchannel plates ͑MCPs͒ as a function of gain, MCP-to-readout distance and accelerating electric field are presented. To investigate the charge footprint variation, we introduce a ballistic model of the charge cloud propagation based on the energy and angular distribution at the MCP(More)
The spatial resolution of position-sensitive detectors that use stacks of microchannel plates (MCP's) with high-resolution anodes can be better than 20-microm FWHM [Proc. SPIE 3114, 283-294 (1997)]. At this level of accuracy, channel misalignments of the MCP's in the stack can cause observable moiré interference patterns. We show that the flat-field(More)
The expanding spectrum of applications of single-molecule fluorescence imaging ranges from fundamental in vitro studies of biomolecular activity to tracking of receptors in live cells. The success of these assays has relied on progress in organic and non-organic fluorescent probe developments as well as improvements in the sensitivity of light detectors. We(More)
The cross-strip imaging readout employs charge division, and centroiding, of microchannel plate charge signals detected on two orthogonal layers of sense strips to encode event X-Y positions and times. We have developed cross-strip detectors and fully parallel channel position encoding electronics. The front-end amplifiers utilize two 32-channel(More)
Fluorescence lifetime imaging (FLIM) is a powerful approach to studying the immediate environment of molecules. For example, it is used in biology to study changes in the chemical environment, or to study binding processes, aggregation, and conformational changes by measuring Förster resonance energy transfer (FRET) between donor and acceptor fluorophores.(More)
A number of modern experiments require simultaneous measurement of charges on multiple channels at > MHz event rates with an accuracy of 100-1000 e(-) rms. One widely used data processing scheme relies on application of specific integrated circuits enabling multichannel analog peak detection asserted by an external trigger followed by a serial/sparsified(More)
Fluorescence lifetime can be used as a contrast mechanism to distinguish fluorophores for localization or tracking, for studying molecular interactions, binding, assembly, and aggregation, or for observing conformational changes via Förster resonance energy transfer (FRET) between donor and acceptor molecules. Fluorescence lifetime imaging microscopy(More)
We have made substantial progress in the development of GaN photocathodes, including crystalline and polycrystalline GaN and InGaN coatings grown by chemical vapor deposition or molecular beam epitaxy on sapphire substrates. GaN and InGaN photocathodes have been developed with efficiencies up to 70% and cutoffs at $380 nm with low out of band response, and(More)
It was shown previously that CsI sensitivity can be substantially reduced by intense UV irradiation. In the present study we measure the influence of negative and positive electric fields on the degradation of CsI photocathode sensitivity when it is subject to a large dose UV irradiation. The UV sensitivity of positively biased CsI photocathodes did not(More)