Anton S. Tremsin

  • Citations Per Year
Learn More
Two optical configurations are commonly used in single-molecule fluorescence microscopy: point-like excitation and detection to study freely diffusing molecules, and wide field illumination and detection to study surface immobilized or slowly diffusing molecules. Both approaches have common features, but also differ in significant aspects. In particular,(More)
Fluorescence lifetime imaging (FLIM) is a powerful approach to studying the immediate environment of molecules. For example, it is used in biology to study changes in the chemical environment, or to study binding processes, aggregation, and conformational changes by measuring Förster resonance energy transfer (FRET) between donor and acceptor fluorophores.(More)
A new hybrid optical detector is described that has many of the attributes desired for the next generation adaptive optics (AO) wavefront sensors. The detector consists of a proximity focused MCP read out by multipixel application specific integrated circuit (ASIC) chips developed at CERN (“Medipix2”) with individual pixels that amplify, discriminate and(More)
The photoemissive stability of as-deposited and heat-treated CsI, KI and KBr evaporated thin "lms under UV radiation is examined in this paper. After the deposition, some photocathodes were annealed for several hours at 903C in vacuum and their performance was then compared to the performance of non-heated samples. We observed that the post-evaporation(More)
Fluorescence lifetime can be used as a contrast mechanism to distinguish fluorophores for localization or tracking, for studying molecular interactions, binding, assembly, and aggregation, or for observing conformational changes via Förster resonance energy transfer (FRET) between donor and acceptor molecules. Fluorescence lifetime imaging microscopy(More)
We studied variation of quantum efficiencies of CsI, KBr and KI-evaporated reflective planar photocathodes with the angle of radiation incidence (±55 degrees) in the spectral range of 25-115 nm. The photocurrent increases with the photon incidence angle for short wavelengths (by as much as ~60% at 55 for 25-30 nm illumination), while it changes only ~5% at(More)
Transmission electron microscopy has been employed to study the structure of polycrystalline CsI thin "lms and its transformation under exposure to humid air and UV irradiation. The catastrophic degradation of CsI thin "lm photocathode performance is shown to be associated with the "lm dissolving followed by its re-crystallization. This results in the(More)
  • X Michalet, R A Colyer, +4 authors S Weiss
  • Current pharmaceutical biotechnology
  • 2009
The expanding spectrum of applications of single-molecule fluorescence imaging ranges from fundamental in vitro studies of biomolecular activity to tracking of receptors in live cells. The success of these assays has relied on progress in organic and non-organic fluorescent probe developments as well as improvements in the sensitivity of light detectors. We(More)
The optical constants of thin films of CsI, KI, and KBr in the spectral range of 53.6-174.4 nm were obtained from the measurements of reflectivity as a function of the incidence angle. The effect of film heating to 420 K and exposure to UV radiation on the optical constants of the three materials was also investigated. The quantum efficiencies (QEs) of the(More)
The measurements of the electron cloud footprints produced by a stack of microchannel plates ~MCPs! as a function of gain, MCP-to-readout distance and accelerating electric field are presented. To investigate the charge footprint variation, we introduce a ballistic model of the charge cloud propagation based on the energy and angular distribution at the MCP(More)